Difference between revisions of "CysJ"

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* '''Mutant:'''
 
* '''Mutant:'''
 +
** 1A802 ( ''cysJ''::''kan''), {{PubMed|11445163}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A802&Search=1A802 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:05, 19 September 2012

  • Description: sulfite reductase (NADPH2)

Gene name cysJ
Synonyms yvgQ
Essential no
Product sulfite reductase (NADPH2)
Function sulfite reduction
Gene expression levels in SubtiExpress: cysJ
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 64 kDa, 5.49
Gene length, protein length 1713 bp, 571 aa
Immediate neighbours nhaK, cysI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvgQ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CysJ expression.png




























Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CysL regulon

The gene

Basic information

  • Locus tag: BSU33430

Phenotypes of a mutant

  • unable to grow with sulfate or sulfite as the only sulfur source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): contains an iron-sulfur cluster
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by sulfite, sulfate, and thiosulfate (CysL) PubMed
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Isabelle Guillouard, Sandrine Auger, Marie-Françoise Hullo, Farid Chetouani, Antoine Danchin, Isabelle Martin-Verstraete
Identification of Bacillus subtilis CysL, a regulator of the cysJI operon, which encodes sulfite reductase.
J Bacteriol: 2002, 184(17);4681-9
[PubMed:12169591] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

J R van der Ploeg, M Barone, T Leisinger
Functional analysis of the Bacillus subtilis cysK and cysJI genes.
FEMS Microbiol Lett: 2001, 201(1);29-35
[PubMed:11445163] [WorldCat.org] [DOI] (P p)