cysI

cysI
168

sulfite reductase (NADPH2) (beta subunit)

locus
BSU_33430
Molecular weight
64.63 kDa
pI
5.49
Protein length
Gene length
function
sulfite reduction
product
sulfite reductase (NADPH2)
essential
no
synonyms
cysI, yvgR

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0155 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,430,598  3,432,313
Phenotypes of a mutant
unable to grow with sulfate or sulfite as the only sulfur source [Pubmed|12169591]
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The protein
Catalyzed reaction/ biological activity
3 H2O + hydrogen sulfide + 3 NADP+ --> 4 H+ + 3 NADPH + sulfite (according to UniProt)
Protein family
nitrite and sulfite reductase 4Fe-4S domain family (with [protein|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD], according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
heme [pubmed|29852252]
Structure
[PDB|6C3M] (from E. coli, 52% identity) [pubmed|29852252]
[AF|O32213]
Expression and Regulation
Operons
genes
[gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]-[gene|5A99F57DF0DDF5A129C3702E97621232A8173C22|cysJ]
description
[Pubmed|12169591]
regulation
induced by sulfite, sulfate, and thiosulfate ([protein|search|CysL]) [Pubmed|12169591]
regulatory mechanism
[protein|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|cysL]: activation, [Pubmed|12169591], in [regulon|protein:FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|cysL regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12169591], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]→[gene|5A99F57DF0DDF5A129C3702E97621232A8173C22|cysJ]

2024-03-27 21:33:23

ghost

51

c76dbdd9ed7d8724551be3211cb722c3be05ea7a

CF5DADBE8969C7BB4BA71235FA6595D0555E76EC

Biological materials
Mutant
MGNA-B602 (yvgR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1601 NBRP B. subtilis, Japan]
1A802 ( ''cysI''::''kan''), [Pubmed|11445163], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A802&Search=1A802 BGSC]
1A934 ( ''cysI''::''kan''), [Pubmed|12169591], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]
BKE33430 (''[gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]''::''erm'', available in the BGSC, in [wiki|Fabian Commichau]'s, and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
BKE33430 ([gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTGAAAAACTCCTTTC,  downstream forward: _UP4_TGAGAAAGAAAGACGAGAGA
BKK33430 ([gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTGAAAAACTCCTTTC,  downstream forward: _UP4_TGAGAAAGAAAGACGAGAGA
References
12169591,12107147,11445163,29852252

A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D

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Time of last update: 2024-03-28 20:01:47

Author of last update: Melvin.boenninger