Difference between revisions of "CysI"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 1772 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 1407 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:26, 17 April 2014

  • Description: sulfite reductase (NADPH2)

Gene name cysI
Synonyms yvgR
Essential no
Product sulfite reductase (NADPH2)
Function sulfite reduction
Gene expression levels in SubtiExpress: cysI
Metabolic function and regulation of this protein in SubtiPathways:
cysI
MW, pI 67 kDa, 4.573
Gene length, protein length 1815 bp, 605 aa
Immediate neighbours cysJ, helD
Sequences Protein DNA DNA_with_flanks
Genetic context
YvgR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CysI expression.png




























Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CysL regulon

The gene

Basic information

  • Locus tag: BSU33440

Phenotypes of a mutant

  • unable to grow with sulfate or sulfite as the only sulfur source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by sulfite, sulfate, and thiosulfate (CysL) PubMed
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1772 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1407 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Isabelle Guillouard, Sandrine Auger, Marie-Françoise Hullo, Farid Chetouani, Antoine Danchin, Isabelle Martin-Verstraete
Identification of Bacillus subtilis CysL, a regulator of the cysJI operon, which encodes sulfite reductase.
J Bacteriol: 2002, 184(17);4681-9
[PubMed:12169591] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

J R van der Ploeg, M Barone, T Leisinger
Functional analysis of the Bacillus subtilis cysK and cysJI genes.
FEMS Microbiol Lett: 2001, 201(1);29-35
[PubMed:11445163] [WorldCat.org] [DOI] (P p)