CypX

• Description: cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase
  
  

Gene name	cypX
Synonyms
Essential	no
Product	cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase
Function	biosynthesis of the extracellular iron chelate pulcherrimin
Gene expression levels in SubtiExpress: cypX
MW, pI	45 kDa, 5.306
Gene length, protein length	1215 bp, 405 aa
Immediate neighbours	yvnA, yvmC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

acquisition of iron, electron transport/ other, iron metabolism

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU35060

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • implicated in the three-step oxidative transformation of the diketopiperazine cyclo-l-leucyl-l-leucyl into pulcherriminic acid PubMed

• Protein family: cytochrome P450 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3NC3 PubMed

• UniProt: O34926

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yvmC-cypX Reference

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Max J Cryle, Stephen G Bell, Ilme Schlichting
Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase.
Biochemistry: 2010, 49(34);7282-96
[PubMed:20690619] [WorldCat.org] [DOI] (I p)

R L Uffen, E Canale-Parola
Synthesis of pulcherriminic acid by Bacillus subtilis.
J Bacteriol: 1972, 111(1);86-93
[PubMed:4204912] [WorldCat.org] [DOI] (P p)

additional paper