CwlT

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  • Description: cell wall hydrolase, C-terminal domain hydrolyzes bond between D-Glu and m-DAP

Gene name cwlT
Synonyms yddH
Essential
Product cell wall hydrolase
Function cell wall metabolism
Gene expression levels in SubtiExpress: cwlT
MW, pI 36 kDa, 8.632
Gene length, protein length 987 bp, 329 aa
Immediate neighbours yddG, yddI
Sequences Protein DNA DNA_with_flanks
Genetic context
YddH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CwlT expression.png
























Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU04970

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nlpC/p60 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • induced by mitomycin C PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Tyler DeWitt, Alan D Grossman
The bifunctional cell wall hydrolase CwlT is needed for conjugation of the integrative and conjugative element ICEBs1 in Bacillus subtilis and B. anthracis.
J Bacteriol: 2014, 196(8);1588-96
[PubMed:24532767] [WorldCat.org] [DOI] (I p)

Tatsuya Fukushima, Toshihiko Kitajima, Hiroyuki Yamaguchi, Qin Ouyang, Kazumi Furuhata, Hiroki Yamamoto, Toshio Shida, Junichi Sekiguchi
Identification and characterization of novel cell wall hydrolase CwlT: a two-domain autolysin exhibiting n-acetylmuramidase and DL-endopeptidase activities.
J Biol Chem: 2008, 283(17);11117-25
[PubMed:18305117] [WorldCat.org] [DOI] (P p)