cwlT

cwlT
168

cell wall hydrolase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer , C-terminal domain hydrolyzes bond between D-Glu and m-DAP

locus
BSU_04970
Molecular weight
36.39 kDa
pI
8.63
Protein length
Gene length
function
conjugative transfer of ICEBs1
product
cell wall hydrolase
synonyms
cwlT, yddH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0791 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
544,022  545,011
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The protein
Catalyzed reaction/ biological activity
hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D--glutamate and  meso-diaminopimelic acid) [Pubmed|18305117]
degradation of gamma-polyglutamic acid [pubmed|29458655]
Protein family
[wiki|Peptidase C40 family] (according to UniProt)
[wiki|Domains]
N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine [Pubmed|18305117]
C-terminal D,L-endopeptidase domain ([wiki|NlpC/P60 domain]) [pubmed|29458655,22139507], cleaves the bond between D--glutamate and  meso-diaminopimelic acid [Pubmed|18305117]
Structure
[PDB|4FDY] (from Staphylococcus aureus, 42% identity) [pubmed|24051416]
[AF|P96645]
Effectors of protein activity
both enzymatic activities are inhibited by interaction with [protein|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] [pubmed|29458655]
[wiki|Localization]
secreted (with signal peptide) [Pubmed|24532767]
may be a lipoprotein, but the lipid anchor is not required for function [Pubmed|24532767]
Expression and Regulation
Operons
genes
[gene|EE2A4068CBD7A2F852E582E2EDA4148381F1CD61|xis]-[gene|2227923C603B290AB276238F789D71584FB07DF3|ydzL]-[gene|34451538F10B4ABCFC8A236C8C905A20D989EBCF|devI]-[gene|C042792D9A643DCFF4A851EFECA3CF2C31E1EDAF|ydcP]-[gene|4BCAA5939CB7F0DF23495BC92C9EF0A0149296A0|ydcQ]-[gene|8E179976C35FE09F92A191D00E497ED4BF28F571|nicK]-[gene|886B2132717BA4C72433ABE351C5791C9DE1432A|S172]-[gene|1699CFBF5400CAF43C6DAE8ADCCE5A360B31F2E9|ydcS]-[gene|9D4918852A1856B1CDD72D49AEA75E51A8602BB6|ydcT]-[gene|62CEABA069CA2970F22B7E0C369D540A2A027A8D|yddA]-[gene|A0AFD543582D2FE8B1139CF69B7B1F7FF0F80265|conB]-[gene|F273A8817E66B44D58FC2BCB6576A30D8616B750|conC]-[gene|F1F898D7A9FDF78D2E5E8F3BF605E0F735D0542A|conD]-[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]-[gene|B3A07E2A61151C33B7C65D1BC58B97BFDB76852D|yddF]-[gene|3356A43B83E2D4D66098F17A751F0912C1553398|conG]-[gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]-[gene|2E4FF80C857B3F40BA835814EC40D6562721AEFE|yddI]-[gene|2FB1C9435AE924F8E450AFA81F800196A871F2D0|yddJ]
description
[pubmed|22383849]
regulatory mechanism
[protein|DD1C8F3A4809785BD6A6047D39B42AB2C605E161|immR]: repression, [Pubmed|17511812], in [regulon|protein:DD1C8F3A4809785BD6A6047D39B42AB2C605E161|immR regulon]
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[gene|EE2A4068CBD7A2F852E582E2EDA4148381F1CD61|xis]->[gene|2FB1C9435AE924F8E450AFA81F800196A871F2D0|yddJ]

2024-03-03 22:58:45

Bzhu

47

9225e26ee70027d168e8ea0db13fb48c93ef1ef8

DE8D2BDC2F23AB45C58B0FE511E7B4816A65A489

Biological materials
Mutant
BKE04970 ([gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC,  downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
BKK04970 ([gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC,  downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
References
18305117,24532767,24051416,29458655,32419322

8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F

Page visits: 3935

Time of last update: 2024-03-28 15:59:48

Author of last update: Jstuelk