cwlT
168
cell wall hydrolase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer , C-terminal domain hydrolyzes bond between D-Glu and m-DAP
locus
BSU_04970
Molecular weight
36.39 kDa
pI
8.63
function
conjugative transfer of ICEBs1
product
cell wall hydrolase
synonyms
cwlT, yddH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0791 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
544,022 545,011
The protein
Catalyzed reaction/ biological activity
hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D--glutamate and meso-diaminopimelic acid) [Pubmed|18305117]
degradation of gamma-polyglutamic acid [pubmed|29458655]
Protein family
[wiki|Peptidase C40 family] (according to UniProt)
[wiki|Domains]
N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine [Pubmed|18305117]
C-terminal D,L-endopeptidase domain ([wiki|NlpC/P60 domain]) [pubmed|29458655,22139507], cleaves the bond between D--glutamate and meso-diaminopimelic acid [Pubmed|18305117]
Structure
[PDB|4FDY] (from Staphylococcus aureus, 42% identity) [pubmed|24051416]
[AF|P96645]
Effectors of protein activity
both enzymatic activities are inhibited by interaction with [protein|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] [pubmed|29458655]
[wiki|Localization]
secreted (with signal peptide) [Pubmed|24532767]
may be a lipoprotein, but the lipid anchor is not required for function [Pubmed|24532767]
Biological materials
Mutant
BKE04970 ([gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
BKK04970 ([gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
References
Page visits: 4076
Time of last update: 2024-04-24 13:40:04
Author of last update: Jstuelk