CwlO

• Description: D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation
  
  

Gene name	cwlO
Synonyms	yzkA, yvcE
Essential	no
Product	endopeptidase-type autolysin
Function	cell wall synthesis, cell elongation
Gene expression levels in SubtiExpress: cwlO
Interactions involving this protein in SubtInteract: CwlO
MW, pI	50 kDa, 5.326
Gene length, protein length	1419 bp, 473 aa
Immediate neighbours	trxB, yvcD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell wall degradation/ turnover

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

• Locus tag: BSU34800

Phenotypes of a mutant

• a cwlO lytE mutant is not viable PubMed
• shorter, fatter cells, this can be rescued by addition of Mg(2+) PubMed

Database entries

• BsubCyc: BSU34800

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase C40 family (according to Swiss-Prot)

• Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

• Kinetic information:

• Domains:
  • C-terminal D,L-endopeptidase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:
  • CwlO requires activation by FtsE-FtsX PubMed
  • activity requires functional Mbl PubMed

• Interactions:
  • FtsX-CwlO, at the membrane PubMed

• Localization:
  • extracellular (signal peptide) PubMed
  • localizes to the outer lateral sidewall of the cell (via the N-terminal domain) PubMed
  • cell membrane in a FtsX-dependent manner PubMed

Database entries

• BsubCyc: BSU34800

• Structure:

• UniProt: P40767

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: cwlO PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed during exponential growth (WalR) PubMed

• Regulatory mechanism:
  • WalR: transcription activation PubMed

• Additional information:
  • The mRNA has a long 5' leader region (187 N). This may indicate RNA-based regulation PubMed
  • the leader mRNA is subject to cleavage by RNase Y, this results in limited expression of cwlO PubMed
  • there is a SigB-dependent anti-sense transcript which is expressed in the stationary phase PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 207 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 200 PubMed

Biological materials

• Mutant: BKE34800 (cwlO::erm, available in the BGSC and in Jörg Stülke's lab)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: