Difference between revisions of "CwlO"

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* '''Description:''' D,L-endopeptidase-type autolysin <br/><br/>
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* '''Description:''' D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation <br/><br/>
  
 
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{| align="right" border="1" cellpadding="2"  
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128,22139507}}
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* a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128,22139507}}
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* shorter, fatter cells {{PubMed|xxx}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 +
** [[CwlO]] requires activation by [[FtsE]]-[[FtsX]] {{PubMed|xxx}}
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
  
 
* '''[[Localization]]:'''  
 
* '''[[Localization]]:'''  
** secreted (according to Swiss-Prot)
 
 
** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
 
** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
** localizes to the lateral sidewall of the cell (via the N-terminal domain) {{PubMed|22139507}}
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** localizes to the outer lateral sidewall of the cell (via the N-terminal domain) {{PubMed|22139507}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 12:02, 16 July 2013

  • Description: D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation

Gene name cwlO
Synonyms yzkA, yvcE
Essential no
Product endopeptidase-type autolysin
Function cell wall synthesis, cell proliferation
Gene expression levels in SubtiExpress: cwlO
MW, pI 50 kDa, 5.326
Gene length, protein length 1419 bp, 473 aa
Immediate neighbours trxB, yvcD
Sequences Protein DNA DNA_with_flanks
Genetic context
YvcE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CwlO expression.png















Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

  • Locus tag: BSU34800

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase C40 family (according to Swiss-Prot)
  • Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

  • Kinetic information:
  • Domains:
    • C-terminal D,L-endopeptidase domain PubMed
  • Modification:
  • Cofactor(s):
  • Localization:
    • extracellular (signal peptide) PubMed
    • localizes to the outer lateral sidewall of the cell (via the N-terminal domain) PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:
    • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Additional reviews: PubMed

Original publications

Additional publications: PubMed

Masayuki Hashimoto, Seika Ooiwa, Junichi Sekiguchi
Synthetic lethality of the lytE cwlO genotype in Bacillus subtilis is caused by lack of D,L-endopeptidase activity at the lateral cell wall.
J Bacteriol: 2012, 194(4);796-803
[PubMed:22139507] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Paola Bisicchia, David Noone, Efthimia Lioliou, Alistair Howell, Sarah Quigley, Thomas Jensen, Hanne Jarmer, Kevin M Devine
The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);180-200
[PubMed:17581128] [WorldCat.org] [DOI] (P p)

Hiroyuki Yamaguchi, Kazumi Furuhata, Tatsuya Fukushima, Hiroki Yamamoto, Junichi Sekiguchi
Characterization of a new Bacillus subtilis peptidoglycan hydrolase gene, yvcE (named cwlO), and the enzymatic properties of its encoded protein.
J Biosci Bioeng: 2004, 98(3);174-81
[PubMed:16233686] [WorldCat.org] [DOI] (P p)