CwlH

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  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name cwlH
Synonyms yqeE
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function cell wall metabolism
Gene expression levels in SubtiExpress: cwlH
MW, pI 27 kDa, 9.998
Gene length, protein length 750 bp, 250 aa
Immediate neighbours yqeF, yqeD
Sequences Protein DNA DNA_with_flanks
Genetic context
CwlH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CwlH expression.png
























Categories containing this gene/protein

cell wall degradation/ turnover, sporulation proteins, membrane proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon

The gene

Basic information

  • Locus tag: BSU25710

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK, GerE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275] [WorldCat.org] [DOI] (P p)

F A Nugroho, H Yamamoto, Y Kobayashi, J Sekiguchi
Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis.
J Bacteriol: 1999, 181(20);6230-7
[PubMed:10515909] [WorldCat.org] [DOI] (P p)