Difference between revisions of "CwlH"

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(Extended information on the protein)
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|style="background:#ABCDEF;" align="center"|'''Function''' || cell wall metabolism
 
|style="background:#ABCDEF;" align="center"|'''Function''' || cell wall metabolism
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU25710 cwlH]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 9.998   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 9.998   

Revision as of 14:02, 7 August 2012

  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name cwlH
Synonyms yqeE
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function cell wall metabolism
Gene expression levels in SubtiExpress: cwlH
MW, pI 27 kDa, 9.998
Gene length, protein length 750 bp, 250 aa
Immediate neighbours yqeF, yqeD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CwlH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CwlH expression.png
























Categories containing this gene/protein

cell wall degradation/ turnover, sporulation proteins, membrane proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon

The gene

Basic information

  • Locus tag: BSU25710

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK, GerE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275] [WorldCat.org] [DOI] (P p)

F A Nugroho, H Yamamoto, Y Kobayashi, J Sekiguchi
Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis.
J Bacteriol: 1999, 181(20);6230-7
[PubMed:10515909] [WorldCat.org] [DOI] (P p)