Difference between revisions of "CwlH"
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|style="background:#ABCDEF;" align="center"|'''Function''' || cell wall metabolism | |style="background:#ABCDEF;" align="center"|'''Function''' || cell wall metabolism | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25710 cwlH] |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 9.998 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 9.998 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqeF]]'', ''[[yqeD]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqeF]]'', ''[[yqeD]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25710 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25710 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25710 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:cwlH_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:cwlH_context.gif]] | ||
Revision as of 14:21, 13 May 2013
- Description: N-acetylmuramoyl-L-alanine amidase
| Gene name | cwlH |
| Synonyms | yqeE |
| Essential | no |
| Product | N-acetylmuramoyl-L-alanine amidase |
| Function | cell wall metabolism |
| Gene expression levels in SubtiExpress: cwlH | |
| MW, pI | 27 kDa, 9.998 |
| Gene length, protein length | 750 bp, 250 aa |
| Immediate neighbours | yqeF, yqeD |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
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Contents
Categories containing this gene/protein
cell wall degradation/ turnover, sporulation proteins, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25710
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
- Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P54450
- KEGG entry: [3]
- E.C. number: 3.5.1.28
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275]
[WorldCat.org]
[DOI]
(P p)
F A Nugroho, H Yamamoto, Y Kobayashi, J Sekiguchi
Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis.
J Bacteriol: 1999, 181(20);6230-7
[PubMed:10515909]
[WorldCat.org]
[DOI]
(P p)
