Difference between revisions of "CwlH"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' [[cwlH]] {{PubMed|10515909}}
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* '''Operon:''' ''[[cwlH]]'' {{PubMed|10515909}}
  
 
* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|10515909}}
 
* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|10515909}}
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** [[GerE]]: transcription activation {{PubMed|10515909}}
 
** [[GerE]]: transcription activation {{PubMed|10515909}}
  
* '''Additional information:'''  
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* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 19:47, 24 November 2009

  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name cwlH
Synonyms yqeE
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function cell wall metabolism
MW, pI 27 kDa, 9.998
Gene length, protein length 750 bp, 250 aa
Immediate neighbours yqeF, yqeD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CwlH context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU25710

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK, GerE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275] [WorldCat.org] [DOI] (P p)

F A Nugroho, H Yamamoto, Y Kobayashi, J Sekiguchi
Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis.
J Bacteriol: 1999, 181(20);6230-7
[PubMed:10515909] [WorldCat.org] [DOI] (P p)