Difference between revisions of "CwlA"

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=Biological materials =
 
=Biological materials =

Revision as of 11:51, 16 April 2012

  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name cwlA
Synonyms
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function minor autolysin
MW, pI 29 kDa, 10.4
Gene length, protein length 816 bp, 272 aa
Immediate neighbours yqxI, yqxH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CwlA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

cell wall degradation/ turnover, Skin element

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU25900

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

A Kuroda, M Imazeki, J Sekiguchi
Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis.
FEMS Microbiol Lett: 1991, 65(1);9-13
[PubMed:1908396] [WorldCat.org] [DOI] (P p)