Difference between revisions of "CtpB"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 
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** substrate binding of CtpB induces opening of the PDZ gate and protease activation {{PubMed|24243021}}
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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** intracellular space between the mother cell and the forespore {{PubMed|24243021}}
  
 
=== Database entries ===
 
=== Database entries ===
  
 
* '''Structure:'''
 
* '''Structure:'''
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** forms a ring-like scaffold with two narrow tunnels for substrate binding {{PubMed|24243021}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O35002 O35002]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O35002 O35002]
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=References=
 
=References=
  
<pubmed>14526016, 17557826, 16818230 15699190,16497325</pubmed>
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<pubmed>14526016, 17557826, 16818230 15699190,16497325 24243021 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:43, 3 December 2013

  • Description: carboxy-terminal processing serine protease, cleaves SpoIVFA, this results in processing of pro-SigK

Gene name ctpB
Synonyms yvjB
Essential no
Product carboxy-terminal processing serine protease,
cleaves SpoIVFA
Function control of SigK activation
Gene expression levels in SubtiExpress: ctpB
MW, pI 52 kDa, 8.641
Gene length, protein length 1440 bp, 480 aa
Immediate neighbours swrAA/2, ftsX
Sequences Protein DNA DNA_with_flanks
Genetic context
YvjB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CtpB expression.png















Categories containing this gene/protein

proteolysis, sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigG regulon

The gene

Basic information

  • Locus tag: BSU35240

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): CtpA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Effectors of protein activity:
    • substrate binding of CtpB induces opening of the PDZ gate and protease activation PubMed
  • Interactions:

Database entries

  • Structure:
    • forms a ring-like scaffold with two narrow tunnels for substrate binding PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: ctpB (according to DBTBS)
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Markus Mastny, Alexander Heuck, Robert Kurzbauer, Anja Heiduk, Prisca Boisguerin, Rudolf Volkmer, Michael Ehrmann, Christopher D A Rodrigues, David Z Rudner, Tim Clausen
CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation in Bacillus subtilis.
Cell: 2013, 155(3);647-58
[PubMed:24243021] [WorldCat.org] [DOI] (I p)

Nathalie Campo, David Z Rudner
SpoIVB and CtpB are both forespore signals in the activation of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2007, 189(16);6021-7
[PubMed:17557826] [WorldCat.org] [DOI] (P p)

Nathalie Campo, David Z Rudner
A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis.
Mol Cell: 2006, 23(1);25-35
[PubMed:16818230] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Qi Pan, Richard Losick, David Z Rudner
A second PDZ-containing serine protease contributes to activation of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2003, 185(20);6051-6
[PubMed:14526016] [WorldCat.org] [DOI] (P p)