Ctc

• Description: general stress protein, similar to ribosomal protein L25
  
  

Gene name	ctc
Synonyms
Essential	no
Product	ribosomal protein
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	21 kDa, 4.216
Gene length, protein length	612 bp, 204 aa
Immediate neighbours	prs, spoVC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00520

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Ctc subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: binds 5S rRNA PubMed

• Localization: in the ribosome (large subunit) PubMed

Database entries

• Structure: 1FEU (from Thermus thermophilus, 32% identity, 53% similarity) PubMed

• UniProt: P14194

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon:
  • gcaD-prs-ctc PubMed
  • ctc PubMed

• Sigma factor:
  • gcaD: SigA PubMed
  • ctc: SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism: alternative sigma factor, SigB PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant: GP500(spc), available in Stülke lab

• Expression vector:
  • expression/ purification with N-terminal His-tag in E. coli: pGP804 (in pWH844), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Stülke lab