Difference between revisions of "CsrA"

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(Biological materials)
(Biological materials)
Line 106: Line 106:
 
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP383, available in [[Stülke]] lab
 
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP383, available in [[Stülke]] lab
 
        
 
        
* '''lacZ fusion:'''
+
* '''lacZ fusion:''' pGP461 (in [[pAC7]]), available in [[Stülke]] lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 18:34, 29 October 2009

  • Description: similar to E. coli carbon storage regulator

Gene name csrA
Synonyms yviG
Essential no
Product unknown
Function unknown
MW, pI 8 kDa, 6.09
Gene length, protein length 222 bp, 74 aa
Immediate neighbours hag, fliW
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CsrA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU35370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: csrA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP469 (spc), available in Stülke lab
  • Expression vector:
    • pGP381 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP383, available in Stülke lab
  • lacZ fusion: pGP461 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Prajna R Kulkarni, Xiaohui Cui, Joshua W Williams, Ann M Stevens, Rahul V Kulkarni
Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri.
Nucleic Acids Res: 2006, 34(11);3361-9
[PubMed:16822857] [WorldCat.org] [DOI] (I e)