CspB

• Description: major cold-shock protein
  
  

Gene name	cspB
Synonyms
Essential	no
Product	major cold-shock protein
Function	RNA chaperone
Gene expression levels in SubtiExpress: cspB
Interactions involving this protein in SubtInteract: CspB
MW, pI	7 kDa, 4.341
Gene length, protein length	201 bp, 67 aa
Immediate neighbours	yhcI, yhcJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

RNA chaperones, cold stress proteins

The gene

Basic information

• Locus tag: BSU09100

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): CspC, CspD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CshB-CspB PubMed

• Localization: cytoplasm (according to Swiss-Prot), cytoplasma, colocalizes with the ribosomes PubMed

Database entries

• Structure:
  • 1CSQ
  • 1NMG (NMR)
  • 3PF4 (in complex with r(GUCUUUA)) PubMed

• UniProt: P32081

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: cspB PubMed

• Expression browser: cspB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced upon cold shock PubMed

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Additional publications: PubMed

Rolf Sachs, Klaas E A Max, Udo Heinemann, Jochen Balbach
RNA single strands bind to a conserved surface of the major cold shock protein in crystals and solution.
RNA: 2012, 18(1);65-76
[PubMed:22128343] [WorldCat.org] [DOI] (I p)

Karen Hunger, Carsten L Beckering, Frank Wiegeshoff, Peter L Graumann, Mohamed A Marahiel
Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis.
J Bacteriol: 2006, 188(1);240-8
[PubMed:16352840] [WorldCat.org] [DOI] (P p)

Michael H Weber, Mohamed A Marahiel
Bacterial cold shock responses.
Sci Prog: 2003, 86(Pt 1-2);9-75
[PubMed:12838604] [WorldCat.org] [DOI] (P p)

Tanja Kaan, Georg Homuth, Ulrike Mäder, Julia Bandow, Thomas Schweder
Genome-wide transcriptional profiling of the Bacillus subtilis cold-shock response.
Microbiology (Reading): 2002, 148(Pt 11);3441-3455
[PubMed:12427936] [WorldCat.org] [DOI] (P p)

Carsten L Beckering, Leif Steil, Michael H W Weber, Uwe Völker, Mohamed A Marahiel
Genomewide transcriptional analysis of the cold shock response in Bacillus subtilis.
J Bacteriol: 2002, 184(22);6395-402
[PubMed:12399512] [WorldCat.org] [DOI] (P p)

Michael H W Weber, Mohamed A Marahiel
Coping with the cold: the cold shock response in the Gram-positive soil bacterium Bacillus subtilis.
Philos Trans R Soc Lond B Biol Sci: 2002, 357(1423);895-907
[PubMed:12171653] [WorldCat.org] [DOI] (P p)

Michael H W Weber, Ingo Fricke, Niclas Doll, Mohamed A Marahiel
CSDBase: an interactive database for cold shock domain-containing proteins and the bacterial cold shock response.
Nucleic Acids Res: 2002, 30(1);375-8
[PubMed:11752341] [WorldCat.org] [DOI] (I p)

M H Weber, C L Beckering, M A Marahiel
Complementation of cold shock proteins by translation initiation factor IF1 in vivo.
J Bacteriol: 2001, 183(24);7381-6
[PubMed:11717297] [WorldCat.org] [DOI] (P p)

M H Weber, A V Volkov, I Fricke, M A Marahiel, P L Graumann
Localization of cold shock proteins to cytosolic spaces surrounding nucleoids in Bacillus subtilis depends on active transcription.
J Bacteriol: 2001, 183(21);6435-43
[PubMed:11591689] [WorldCat.org] [DOI] (P p)

T Schindler, P L Graumann, D Perl, S Ma, F X Schmid, M A Marahiel
The family of cold shock proteins of Bacillus subtilis. Stability and dynamics in vitro and in vivo.
J Biol Chem: 1999, 274(6);3407-13
[PubMed:9920884] [WorldCat.org] [DOI] (P p)

P L Graumann, M A Marahiel
Cold shock proteins CspB and CspC are major stationary-phase-induced proteins in Bacillus subtilis.
Arch Microbiol: 1999, 171(2);135-8
[PubMed:9914312] [WorldCat.org] [DOI] (P p)

T Schindler, D Perl, P Graumann, V Sieber, M A Marahiel, F X Schmid
Surface-exposed phenylalanines in the RNP1/RNP2 motif stabilize the cold-shock protein CspB from Bacillus subtilis.
Proteins: 1998, 30(4);401-6
[PubMed:9533624] [WorldCat.org] [DOI] (P p)

P Graumann, T M Wendrich, M H Weber, K Schröder, M A Marahiel
A family of cold shock proteins in Bacillus subtilis is essential for cellular growth and for efficient protein synthesis at optimal and low temperatures.
Mol Microbiol: 1997, 25(4);741-56
[PubMed:9379903] [WorldCat.org] [DOI] (P p)

P Graumann, K Schröder, R Schmid, M A Marahiel
Cold shock stress-induced proteins in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4611-9
[PubMed:8755892] [WorldCat.org] [DOI] (P p)

K Schröder, P Graumann, A Schnuchel, T A Holak, M A Marahiel
Mutational analysis of the putative nucleic acid-binding surface of the cold-shock domain, CspB, revealed an essential role of aromatic and basic residues in binding of single-stranded DNA containing the Y-box motif.
Mol Microbiol: 1995, 16(4);699-708
[PubMed:7476164] [WorldCat.org] [DOI] (P p)

G I Makhatadze, M A Marahiel
Effect of pH and phosphate ions on self-association properties of the major cold-shock protein from Bacillus subtilis.
Protein Sci: 1994, 3(11);2144-7
[PubMed:7703860] [WorldCat.org] [DOI] (P p)

P Graumann, M A Marahiel
The major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides.
FEBS Lett: 1994, 338(2);157-60
[PubMed:8307174] [WorldCat.org] [DOI] (P p)

K Schröder, P Zuber, G Willimsky, B Wagner, M A Marahiel
Mapping of the Bacillus subtilis cspB gene and cloning of its homologs in thermophilic, mesophilic and psychrotrophic bacilli.
Gene: 1993, 136(1-2);277-80
[PubMed:8294017] [WorldCat.org] [DOI] (P p)

A Schnuchel, R Wiltscheck, M Czisch, M Herrler, G Willimsky, P Graumann, M A Marahiel, T A Holak
Structure in solution of the major cold-shock protein from Bacillus subtilis.
Nature: 1993, 364(6433);169-71
[PubMed:8321289] [WorldCat.org] [DOI] (P p)

H Schindelin, M A Marahiel, U Heinemann
Universal nucleic acid-binding domain revealed by crystal structure of the B. subtilis major cold-shock protein.
Nature: 1993, 364(6433);164-8
[PubMed:8321288] [WorldCat.org] [DOI] (P p)

G Willimsky, H Bang, G Fischer, M A Marahiel
Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures.
J Bacteriol: 1992, 174(20);6326-35
[PubMed:1400185] [WorldCat.org] [DOI] (P p)

H Schindelin, M Herrler, G Willimsky, M A Marahiel, U Heinemann
Overproduction, crystallization, and preliminary X-ray diffraction studies of the major cold shock protein from Bacillus subtilis, CspB.
Proteins: 1992, 14(1);120-4
[PubMed:1409560] [WorldCat.org] [DOI] (P p)