Difference between revisions of "CshA"

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* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' cytoplasma, colocalizes with the ribosomes [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed]
+
* '''Localization:''' cytoplasm (according to Swiss-Prot),  cytoplasma, colocalizes with the ribosomes [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 14:32, 30 April 2009

  • Description: DEAD-box RNA helicase

Gene name cshA
Synonyms ydbR
Essential no
Product DEAD-box RNA helicase
Function RNA helicase
MW, pI 57 kDa, 9.89
Gene length, protein length 1533 bp, 511 aa
Immediate neighbours murF, ydbS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YdbR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RNA helicase
  • Protein family: DEAD-box RNA helicase

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot), cytoplasma, colocalizes with the ribosomes PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

Your additional remarks

References

  1. Hunger, K., Beckering, C. L., Wiegeshoff, F., Graumann, P. L. & Marahiel, M. A. (2006). Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis. J Bacteriol. 188:240-248. PubMed
  2. Ando, Y. and K. Nakamura (2006) Bacillus subtilis DEAD protein YdbR possesses ATPase, RNA binding, and RNA unwinding activities.Biosci. Biotechnol. Biochem. 70: 1606-1615. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed