crh

crh
168

Carbon-flux regulating HPr, formerly known as Catabolite repression HPr-like protein, control of flux through the harmful methylglyoxal pathway, minor cofactor of the [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA] transcription factor

locus
BSU_34740
Molecular weight
9.19 kDa
pI
4.7
Protein length
Gene length
function
control of carbon flux
product
carbon-flux regulating HPr
essential
no
synonyms
crh, yvcM

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1925 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,569,292  3,569,549
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The protein
Protein family
HPr family (with [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH], according to UniProt)
[wiki|Domains]
Hpr domain (aa 1-85) (according to UniProt)
Structure
[PDB|2AK7] (dimeric Crh-Ser46-P) [pubmed|16411239]
[PDB|1ZVV] ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]-Crh-DNA complex)
[PDB|2RLZ] (dimer)
[PDB|1MU4]
[AF|O06976]
Modification
phosphorylation on Ser46 by [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|hprK] [Pubmed|9237995,21992469,22092971]
Paralogous protein(s)
[protein|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]
Additional information
Crh does not possess the phosphorylation site used for [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI] phosphotransfer (His-15 in [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]), it can only be phosphorylated on Ser-46
Expression and Regulation
Operons
genes
[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
description
[Pubmed|9237995]
regulation
constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]
expression
in Enterococcus faecalis: induced upon cell wall stress (vancomycin) ([protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]) [pubmed|38512984]
regulatory mechanism
[protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]: transcription repression, described for Enterococcus faecalis [pubmed|38512984], in [regulon|protein:1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16272399], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB]' [PubMed|20525796]
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[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]→[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]

2024-03-22 09:41:40

Jstuelk

98

BCC5699F6256B8A1B7FD8A979DBBBC9D4D1198AB

9244FB113E998572106D983A19AB3AC81B9F9D35

Biological materials
Mutant
GP860 (Δ[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]::aphA3) [Pubmed|21992469], available in [wiki|Jörg Stülke]'s lab
QB7097 (spc), available in [wiki|Jörg Stülke]'s lab
BKE34740 (Δ[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT,  downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT
BKK34740 (Δ[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT,  downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT
Expression vectors
pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP382]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh], crh(Ser46Asp), crh(Ser46Ala) B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
see [gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]
labs
[wiki|Anne Galinier], University of Marseille, France
[wiki|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
References
12972249,9973552,9237995,16272399,15126459,10217795,17142398,16316990,12009882,10589728,12670692,11916384,11361076,11361074,18320329,18284240,16411239,21992469,22092971,29514981

A269774F2FDC94F93BA5F1360FFFE754B50383AD

Page visits: 6266

Time of last update: 2024-03-28 04:42:22

Author of last update: Melvin.boenninger