cotH

168

protein kinase, required for the assembly of several outer coat proteins

locus: BSU_36060

Molecular weight: 42.65 kDa

pI: 5.95

Protein length: 362 aa

Gene length: 1089 bp

function: protection of [protein|EB690FA075EAC3E298C47AED082B5FC21E8F8F46|cotU] and [protein|9C59B51FC19FC83BD14272008BD441833DD1738E|cotC] in the mother cell

product: protein kinase

essential: no

synonyms: cotH, ywrH

List of homologs in different organisms, belongs to COG5337 (Galperin et al., 2021)

Gene

Coordinates: 3,716,009 → 3,717,097

Phenotypes of a mutant

• overexpression of ''cotH'' suppresses the phenotypes of a [gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] mutant [Pubmed|24086406]
• reduced [wiki|germination] efficiency [Pubmed|27185916]

Advertisement

The protein

Catalyzed reaction/ biological activity

• autophosphorylation with ATP and subsequent phosphorylation of [protein|2A0B2CA7C982C03C1AFBDB3540A918EE937B06CB|cotB] and [protein|49BAFE3E69F1F91AEF4154F55B44639DD3CE4A71|cotG] on serine residues [Pubmed|32592201,27185916]

Protein family

• cotH family (with [protein|75FBA935A39AF67DB4E69C8699D29D9F5C149143|yisJ], according to UniProt)

[wiki|Cofactors]

• Mn²⁺ [Pubmed|27185916]

Structure

• [PDB|5JD9] [Pubmed|27185916]
• [AF|Q45535]

[wiki|Localization]

• inner spore coat

Biological materials

Mutant

• BKE36060 (Δ[gene|D53067098F693669F61E5CCF6C06664580C40C79|cotH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCATAATCCTCCTTACA,  downstream forward: _UP4_TGAATGCGTGAAAATGGGTA
• BKK36060 (Δ[gene|D53067098F693669F61E5CCF6C06664580C40C79|cotH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCATAATCCTCCTTACA,  downstream forward: _UP4_TGAATGCGTGAAAATGGGTA

References

Reviews

• 27227299,34500011

Original Publications

• 10198031,15699190,8755863,15470121,18065538,24086406,21984783,25259857,25115591,26484546,26953338,27185916,14762006,26577401,28870294,31713316,32592201,33596081,35254711