CotE

• Description: spore coat protein (outer)
  
  

Gene name	cotE
Synonyms
Essential	no
Product	spore coat protein (outer)
Function	assembly of CotC-CotU complex
Interactions involving this protein in SubtInteract: CotE
MW, pI	20 kDa, 4.139
Gene length, protein length	543 bp, 181 aa
Immediate neighbours	ymcA, mutS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU17030

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: assembly of the CotC-CotU complex PubMed

• Protein family: cotE family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: homotypic interaction PubMed, CotE-CotC PubMed, CotE-CotU PubMed

• Localization: spore wall (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P14016

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotE PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • expressed during sporulation in the mother cell (SigE, SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Arthur Aronson, Purdue University, West Lafayette, USA homepage

Adriano Henriques, Lisbon, Portugal homepage

References

Jose-Luis Sanchez-Salas, Barbara Setlow, Pengfei Zhang, Yong-Qing Li, Peter Setlow
Maturation of released spores is necessary for acquisition of full spore heat resistance during Bacillus subtilis sporulation.
Appl Environ Microbiol: 2011, 77(19);6746-54
[PubMed:21821751] [WorldCat.org] [DOI] (I p)

Rachele Isticato, Assunta Pelosi, Maurilio De Felice, Ezio Ricca
CotE binds to CotC and CotU and mediates their interaction during spore coat formation in Bacillus subtilis.
J Bacteriol: 2010, 192(4);949-54
[PubMed:20023017] [WorldCat.org] [DOI] (I p)

Daniela Krajcíková, Magda Lukácová, Denisa Müllerová, Simon M Cutting, Imrich Barák
Searching for protein-protein interactions within the Bacillus subtilis spore coat.
J Bacteriol: 2009, 191(10);3212-9
[PubMed:19304857] [WorldCat.org] [DOI] (I p)

Rachele Isticato, Assunta Pelosi, Rita Zilhão, Loredana Baccigalupi, Adriano O Henriques, Maurilio De Felice, Ezio Ricca
CotC-CotU heterodimerization during assembly of the Bacillus subtilis spore coat.
J Bacteriol: 2008, 190(4);1267-75
[PubMed:18065538] [WorldCat.org] [DOI] (I p)

Caitlin C Ferguson, Amy H Camp, Richard Losick
gerT, a newly discovered germination gene under the control of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2007, 189(21);7681-9
[PubMed:17720779] [WorldCat.org] [DOI] (P p)

Teresa Costa, Mónica Serrano, Leif Steil, Uwe Völker, Charles P Moran, Adriano O Henriques
The timing of cotE expression affects Bacillus subtilis spore coat morphology but not lysozyme resistance.
J Bacteriol: 2007, 189(6);2401-10
[PubMed:17172339] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Katerina Ragkousi, Patrick Eichenberger, Christiaan van Ooij, Peter Setlow
Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate.
J Bacteriol: 2003, 185(7);2315-29
[PubMed:12644503] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

S Little, A Driks
Functional analysis of the Bacillus subtilis morphogenetic spore coat protein CotE.
Mol Microbiol: 2001, 42(4);1107-20
[PubMed:11737650] [WorldCat.org] [DOI] (P p)

T Bauer, S Little, A G Stöver, A Driks
Functional regions of the Bacillus subtilis spore coat morphogenetic protein CotE.
J Bacteriol: 1999, 181(22);7043-51
[PubMed:10559171] [WorldCat.org] [DOI] (P p)

F Ginetti, M Perego, A M Albertini, A Galizzi
Bacillus subtilis mutS mutL operon: identification, nucleotide sequence and mutagenesis.
Microbiology (Reading): 1996, 142 ( Pt 8);2021-9
[PubMed:8760914] [WorldCat.org] [DOI] (P p)

K Pogliano, E Harry, R Losick
Visualization of the subcellular location of sporulation proteins in Bacillus subtilis using immunofluorescence microscopy.
Mol Microbiol: 1995, 18(3);459-70
[PubMed:8748030] [WorldCat.org] [DOI] (P p)

C D Webb, A Decatur, A Teleman, R Losick
Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5906-11
[PubMed:7592342] [WorldCat.org] [DOI] (P p)

A Driks, S Roels, B Beall, C P Moran, R Losick
Subcellular localization of proteins involved in the assembly of the spore coat of Bacillus subtilis.
Genes Dev: 1994, 8(2);234-44
[PubMed:8299942] [WorldCat.org] [DOI] (P p)

L B Zheng, R Losick
Cascade regulation of spore coat gene expression in Bacillus subtilis.
J Mol Biol: 1990, 212(4);645-60
[PubMed:1691789] [WorldCat.org] [DOI] (P p)

L B Zheng, W P Donovan, P C Fitz-James, R Losick
Gene encoding a morphogenic protein required in the assembly of the outer coat of the Bacillus subtilis endospore.
Genes Dev: 1988, 2(8);1047-54
[PubMed:3139490] [WorldCat.org] [DOI] (P p)