ComS

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  • Description: antiadaptor protein, assembly link between regulatory components of the competence signal transduction pathway

Gene name comS
Synonyms
Essential no
Product antiadaptor protein (anti-MecA)
Function control of ComK degradation
Gene expression levels in SubtiExpress: comS
Interactions involving this protein in SubtInteract: ComS
MW, pI 5 kDa, 9.145
Gene length, protein length 138 bp, 46 aa
Immediate neighbours srfAB, srfAC
Sequences Protein DNA DNA_with_flanks
Genetic context
ComS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ComS expression.png















Categories containing this gene/protein

genetic competence, short peptides

This gene is a member of the following regulons

Abh regulon, CodY regulon, ComA regulon, PerR regulon, Spx regulon

The gene

Basic information

  • Locus tag: BSU03500

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • repressed during hydrogen peroxide stress (PerR) PubMed
    • expressed at high cell density (ComA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Janine Kirstein, Noël Molière, David A Dougan, Kürşad Turgay
Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases.
Nat Rev Microbiol: 2009, 7(8);589-99
[PubMed:19609260] [WorldCat.org] [DOI] (I p)


Original Publications

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Peter Prepiak, David Dubnau
A peptide signal for adapter protein-mediated degradation by the AAA+ protease ClpCP.
Mol Cell: 2007, 26(5);639-47
[PubMed:17560370] [WorldCat.org] [DOI] (P p)

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Shunji Nakano, Michiko M Nakano, Ying Zhang, Montira Leelakriangsak, Peter Zuber
A regulatory protein that interferes with activator-stimulated transcription in bacteria.
Proc Natl Acad Sci U S A: 2003, 100(7);4233-8
[PubMed:12642660] [WorldCat.org] [DOI] (P p)

M M Nakano, F Hajarizadeh, Y Zhu, P Zuber
Loss-of-function mutations in yjbD result in ClpX- and ClpP-independent competence development of Bacillus subtilis.
Mol Microbiol: 2001, 42(2);383-94
[PubMed:11703662] [WorldCat.org] [DOI] (P p)

M Persuh, K Turgay, I Mandic-Mulec, D Dubnau
The N- and C-terminal domains of MecA recognize different partners in the competence molecular switch.
Mol Microbiol: 1999, 33(4);886-94
[PubMed:10447896] [WorldCat.org] [DOI] (P p)

M Ogura, L Liu, M Lacelle, M M Nakano, P Zuber
Mutational analysis of ComS: evidence for the interaction of ComS and MecA in the regulation of competence development in Bacillus subtilis.
Mol Microbiol: 1999, 32(4);799-812
[PubMed:10361283] [WorldCat.org] [DOI] (P p)

K Turgay, L W Hamoen, G Venema, D Dubnau
Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis.
Genes Dev: 1997, 11(1);119-28
[PubMed:9000055] [WorldCat.org] [DOI] (P p)

J Hahn, A Luttinger, D Dubnau
Regulatory inputs for the synthesis of ComK, the competence transcription factor of Bacillus subtilis.
Mol Microbiol: 1996, 21(4);763-75
[PubMed:8878039] [WorldCat.org] [DOI] (P p)

L W Hamoen, H Eshuis, J Jongbloed, G Venema, D van Sinderen
A small gene, designated comS, located within the coding region of the fourth amino acid-activation domain of srfA, is required for competence development in Bacillus subtilis.
Mol Microbiol: 1995, 15(1);55-63
[PubMed:7752896] [WorldCat.org] [DOI] (P p)

M M Nakano, L A Xia, P Zuber
Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis.
J Bacteriol: 1991, 173(17);5487-93
[PubMed:1715856] [WorldCat.org] [DOI] (P p)