comK
168
competence transcription factor (CTF)
locus
BSU_10420
Molecular weight
22.28 kDa
pI
7.67
function
regulation of [wiki|genetic competence] and DNA uptake
product
competence transcription factor (CTF)
essential
no
synonyms
comK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4903 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,117,109 1,117,687
Phenotypes of a mutant
no expression of competence genes, loss of [wiki|genetic competence] [Pubmed|7783616]
The protein
Structure
[AF|P40396]
Modification
phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 [Pubmed|22517742]
Expression and Regulation
Operons
genes
[gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]
description
[Pubmed|8196543]
regulation
[gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK] has a bistable expression pattern [Pubmed|26110430]
expression is reduced in a [gene|search|cwlO ]mutant [pubmed|29553055]
regulatory mechanism
[protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|11849533], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|8830686], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|12586407], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, (autoregulation) [Pubmed|8083168], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]-P, acts as antagonist of [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok] [Pubmed|22412392], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8196543], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
full expression of [gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK] requires fully assembled and rotating flagella [pubmed|28800172]
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P represses comK expression [pubmed|28800172]
Biological materials
Mutant
1A871 (no resistance), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A871&Search=1A871 BGSC]
BKE10420 ([gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT
BKK10420 ([gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
labs
[wiki|Oscar Kuipers], University of Groningen, The Netherlands
[http://molgen.biol.rug.nl/molgen/index.php Homepage]
References
Reviews
Original Publications
The [wiki|ComK regulon]
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Time of last update: 2024-03-29 05:10:35
Author of last update: Jstuelk