comK

168

competence transcription factor (CTF)

locus: BSU_10420

Molecular weight: 22.28 kDa

pI: 7.67

Protein length: 192 aa

Gene length: 579 bp

function: regulation of [wiki|genetic competence] and DNA uptake

product: competence transcription factor (CTF)

essential: no

synonyms: comK

List of homologs in different organisms, belongs to COG4903 (Galperin et al., 2021)

Gene

Coordinates: 1,117,109  1,117,687

Phenotypes of a mutant

• no expression of competence genes, loss of [wiki|genetic competence] [Pubmed|7783616]

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The protein

Structure

• [AF|P40396]

Modification

• phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 [Pubmed|22517742]

Expression and Regulation

additional information

• full expression of [gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK] requires fully assembled and rotating flagella [pubmed|28800172]
• [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P represses comK expression [pubmed|28800172]

Biological materials

Mutant

• 1A871 (no resistance), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A871&Search=1A871 BGSC]
• BKE10420 ([gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT,  downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT
• BKK10420 ([gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT,  downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

labs

• [wiki|Oscar Kuipers], University of Groningen, The Netherlands
• [http://molgen.biol.rug.nl/molgen/index.php Homepage]

References

Reviews

• 19995980,12576575,26110607,31950915,34438398

Original Publications

• 8878039,11849533,17569828,9335269,17560370,12586407,11814663,9108277,9890793,9585513,15819619,10908654,9000055,7746142,8830686,10447896,12028382,8016067,10361283,11703662,12107147,17581123,8016066,17493123,16391071,16436435,12270822,15598897,20300532,12761164,12036071,21085679,22517742,7783616,22412392,8083168,24838881,8196543,26110430,27045827,27920766,28800172,29124898,29553055,34016970

The [wiki|ComK regulon]

• 11948146,11918817