comK
168
competence transcription factor (CTF)
locus
BSU_10420
Molecular weight
22.28 kDa
pI
7.67
function
regulation of [wiki|genetic competence] and DNA uptake
product
competence transcription factor (CTF)
essential
no
synonyms
comK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4903 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,117,109 1,117,687
Phenotypes of a mutant
no expression of competence genes, loss of [wiki|genetic competence] [Pubmed|7783616]
The protein
Structure
[AF|P40396]
Modification
phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 [Pubmed|22517742]
Expression and Regulation
additional information
full expression of [gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK] requires fully assembled and rotating flagella [pubmed|28800172]
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P represses comK expression [pubmed|28800172]
Biological materials
Mutant
1A871 (no resistance), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A871&Search=1A871 BGSC]
BKE10420 ([gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT
BKK10420 ([gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
labs
[wiki|Oscar Kuipers], University of Groningen, The Netherlands
[http://molgen.biol.rug.nl/molgen/index.php Homepage]
References
Reviews
Original Publications
The [wiki|ComK regulon]
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Time of last update: 2024-04-24 13:23:24
Author of last update: Jstuelk