comGA

168

late competence protein, traffic ATPase, binds and transports transforming DNA, required for growth arrest of competent cells

locus: BSU_24730

Molecular weight: 40.30 kDa

pI: 8.89

Protein length: 356 aa

Gene length: 1071 bp

function: genetic competence

product: traffic ATPase

essential: no

synonyms: comGA

List of homologs in different organisms, belongs to COG2804 (Galperin et al., 2021)

Gene

Coordinates: 2,559,007  2,560,077

Phenotypes of a mutant

• loss of [wiki|genetic competence]

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The protein

Catalyzed reaction/ biological activity

• binds and transports transforming DNA
• sequesters [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] during expression of [Pubmed|genetic competence] to inhibit cell elongation during escape from competence [Pubmed|26091431]

Protein family

• GSP E family (single member, according to UniProt)

Structure

• [PDB|4PHT] (from Vibrio vulnificus, 38% identity) [pubmed|25092625]
• [AF|P25953]

Modification

• phosphorylated on Arg-28, Arg-71- and Arg-330 [Pubmed|22517742]

[wiki|Localization]

• peripheral membrane protein [Pubmed|9723928]
• localizes to cell poles in competent cells [Pubmed|16009133]
• localizes to one cell pole, this depends on [protein|954359717E15DFC106A9D8111C6637E46B16F8C9|comEB] [Pubmed|31954084,21278288]

Additional information

• ComGA stability depends on [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|21564336]

Biological materials

Mutant

• BKE24730 ([gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTTGATTCCCTCTCCTT,  downstream forward: _UP4_TAGAAAAGTCTGGTTGTTAA
• BKK24730 ([gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTTGATTCCCTCTCCTT,  downstream forward: _UP4_TAGAAAAGTCTGGTTGTTAA
• BP914 (([gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-[gene|EA1C841FC77A1994A85E2E0C0A554482C3EC9323|comGB]-[gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]-[gene|D323AEF5AFB4FD2A074ABAB76A39BE26A4C6436F|comGD]-[gene|FF4DB7FDCEB440064E11E65CB0DBFB93FC785CF0|comGE]-[gene|DD266DC0D78B5C5E82EED7CC7F55D0CF8FB4BF7A|comGF]-[gene|89442BFDFBFF264D0C7F12DEA6F8130C55D1F3F8|comGG])::phleo), available in [wiki|Fabian Commichau]'s lab

GFP fusion

• GP1640 ''trpC2'' ''[gene|154862E3FFA7CEBA5725BAD08E09D0E1C6B7F946|ymdB]''::''spc'' P''comG-gfp'' (''cat''), available in [wiki|Jörg Stülke]'s lab
• GP1643 P''comG-gfp'' (''cat'') in the NCIB3610 background, available in [wiki|Jörg Stülke]'s lab
• PG389 [gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::(p[gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-lacZ gfp cat) [pubmed|26110430], available in [wiki|Leendert Hamoen]'s and [wiki|Jörg Stülke]'s labs

References

Reviews

• 15083159,31950915

Original Publications

• 11814663,9422590,16009133,11298275,17630974,9723928,21278288,12107147,8196543,2507524,21564336,21707789,22517742,25899641,26091431,25092625,31954084,34126763,37527273