Difference between revisions of "ComGA"

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(Biological materials)
(Biological materials)
Line 142: Line 142:
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  
* '''GFP fusion:''' GP1640 ''trpC2'' ''[[tkmA]]''::spc ''comG-gfp'' (spc), available in [[ Jörg Stülke]]'s lab
+
* '''GFP fusion:'''  
 +
GP1640 ''trpC2'' ''[[ymdB]]''::spc ''comG-gfp'' (''spc''), available in [[ Jörg Stülke]]'s lab
 +
GP1643 ''comG-gfp'' (''spc'') in the NCIB3610 background, available in [[ Jörg Stülke]]'s lab
  
 
* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  

Revision as of 15:46, 3 February 2015

  • Description: late competence protein, traffic ATPase, binds and transports transforming DNA

Gene name comGA
Synonyms
Essential no
Product traffic ATPase
Function genetic competence
Gene expression levels in SubtiExpress: comGA
Interactions involving this protein in SubtInteract: ComGA
Metabolic function and regulation of this protein in SubtiPathways:
ComGA
MW, pI 40 kDa, 8.893
Gene length, protein length 1068 bp, 356 aa
Immediate neighbours comGB, corA
Sequences Protein DNA DNA_with_flanks
Genetic context
ComGA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ComGA expression.png
























Categories containing this gene/protein

genetic competence, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU24730

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds and transports transforming DNA
  • Protein family: GSP E family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-28, Arg-71- and Arg-330 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:

GP1640 trpC2 ymdB::spc comG-gfp (spc), available in Jörg Stülke's lab GP1643 comG-gfp (spc) in the NCIB3610 background, available in Jörg Stülke's lab

  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)

Original publications