comFA

168

membrane-associated DNA-dependent ATPase, may provide energy for DNA transport

locus: BSU_35470

Molecular weight: 52.40 kDa

pI: 9.82

Protein length: 463 aa

Gene length: 1392 bp

function: genetic competence

product: ATP-binding protein

essential: no

synonyms: comFA

List of homologs in different organisms, belongs to COG4098 (Galperin et al., 2021)

Gene

Coordinates: 3,642,167  3,643,558

Phenotypes of a mutant

• knockout has 1000-fold reduction in transformability compared to the wild-type [Pubmed|8412657]

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The protein

Catalyzed reaction/ biological activity

• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• [wiki|helicase family] (according to UniProt)

[wiki|Domains]

• [wiki|Helicase ATP-binding domain] (aa 133-285) (according to UniProt)
• [wiki|Helicase C-terminal domain] (aa 317-463) (according to UniProt)

[wiki|Cofactors]

• Zn²⁺ [pubmed|28559293]

Structure

• [AF|P39145]

Modification

• phosphorylated on Arg-20, Arg-186, and Arg-446 [Pubmed|22517742]

[wiki|Localization]

• cell membrane [Pubmed|7984101]
• localizes to cell poles in competent cells [Pubmed|16009133]
• localizes to cell poles [Pubmed|21278288]
• localizes along the long axis of competent cells [pubmed|37292776]

Biological materials

Mutant

• BKE35470 ([gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACATAGCACGCCTCCTTTC,  downstream forward: _UP4_TAGCCATTATTTGAAAACGT
• BKK35470 ([gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACATAGCACGCCTCCTTTC,  downstream forward: _UP4_TAGCCATTATTTGAAAACGT

References

Reviews

• 15083159,31950915

Original Publications

• 11814663,8412657,7984101,16009133,8045895,17630974,21278288,22517742,21397556,28559293,28618091,34126763,36815589,37292776