comEC

168

channel protein for DNA binding and uptake

locus: BSU_25570

Molecular weight: 86.51 kDa

pI: 6.96

Protein length: 776 aa

Gene length: 2331 bp

function: genetic competence

product: DNA channel

essential: no

synonyms: comEC, comD

List of homologs in different organisms, belongs to COG2333 (Galperin et al., 2021)

Gene

Coordinates: 2,637,543  2,639,873

Phenotypes of a mutant

• loss of [wiki|genetic competence] [pubmed|33897624]

Advertisement

The protein

Catalyzed reaction/ biological activity

• extracellular degradation of one strand of the dsDNA and uptake of the remaining ssDNA molecule [pubmed|33772889]

[wiki|Domains]

• N-terminal transmembrane DNA-transport channel
• C-terminal extracellular nuclease domain [pubmed|33772889]

[wiki|Cofactors]

• Mn²⁺ (for nuclease activity) [pubmed|33772889]

Structure

• [AF|P39695]

[wiki|Localization]

• cell membrane [Pubmed|15661011]
• localizes to one or both cell poles [Pubmed|34928178,21278288]

Expression and Regulation

Other regulations

• [protein|F21A75744FA0B25D5A251CB57E7A7DC6ABFF1DC7|comN]: unknown, [pubmed|19028902]

additional information

• translation of [protein|363C6FE07C5E56FDE6BEC6ACFBA1A5535F0F64AC|comEC] is translationally coupled to expression of [protein|954359717E15DFC106A9D8111C6637E46B16F8C9|comEB] [pubmed|33527432]

Biological materials

Mutant

• GP2643 (Δ[gene|363C6FE07C5E56FDE6BEC6ACFBA1A5535F0F64AC|comEC]::spc) available in [wiki|Jörg Stülke]'s lab [pubmed|33897624]
• BKE25570 ([gene|363C6FE07C5E56FDE6BEC6ACFBA1A5535F0F64AC|comEC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCATCACACGTAGCTCG,  downstream forward: _UP4_TAAAAAAGACTGCCGAGAAA
• BKK25570 ([gene|363C6FE07C5E56FDE6BEC6ACFBA1A5535F0F64AC|comEC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCATCACACGTAGCTCG,  downstream forward: _UP4_TAAAAAAGACTGCCGAGAAA

References

Reviews

• 15083159,31950915

Original Publications

• 17630974,11814663,15661011,7968523,21278288,19028902,27318187,33527432,33772889,33897624,34126763,34928178,36815589