Difference between revisions of "ComEA"

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(Extended information on the protein)
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* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
 
** cell membrane, no distinct position {{PubMed|21278288}}
 
** cell membrane, no distinct position {{PubMed|21278288}}
 +
** integral membrane protein {{PubMed|24164455}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 12:01, 16 March 2014

  • Description: membrane DNA receptor, plays an important role in the transfer of transforming DNA into the DNA channel and in controlling the rate of DNA uptake

Gene name comEA
Synonyms comD
Essential no
Product membrane DNA receptor
Function genetic competence
Gene expression levels in SubtiExpress: comEA
Interactions involving this protein in SubtInteract: ComEA
Metabolic function and regulation of this protein in SubtiPathways:
comEA
MW, pI 21 kDa, 4.824
Gene length, protein length 615 bp, 205 aa
Immediate neighbours comEB, comER
Sequences Protein DNA DNA_with_flanks
Genetic context
ComEA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ComEA expression.png















Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

ComK regulon, ComN regulon

The gene

Basic information

  • Locus tag: BSU25590

Phenotypes of a mutant

  • strongly reduced transformation rate PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • extracellular DNA-binding domain
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2DUY (from Thermus thermophilus hb8, 37% identity, 60% similarity)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Masaomi Takeno, Hisataka Taguchi, Takashi Akamatsu
Role of ComEA in DNA uptake during transformation of competent Bacillus subtilis.
J Biosci Bioeng: 2012, 113(6);689-93
[PubMed:22398145] [WorldCat.org] [DOI] (I p)

Kenneth Briley, Angella Dorsey-Oresto, Peter Prepiak, Miguel J Dias, Jessica M Mann, David Dubnau
The secretion ATPase ComGA is required for the binding and transport of transforming DNA.
Mol Microbiol: 2011, 81(3);818-30
[PubMed:21707789] [WorldCat.org] [DOI] (I p)

Miriam Kaufenstein, Martin van der Laan, Peter L Graumann
The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex.
J Bacteriol: 2011, 193(7);1633-42
[PubMed:21278288] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura, Teruo Tanaka
The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD).
J Bacteriol: 2009, 191(3);949-58
[PubMed:19028902] [WorldCat.org] [DOI] (I p)

Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974] [WorldCat.org] [DOI] (P p)

Inês Chen, Roberta Provvedi, David Dubnau
A macromolecular complex formed by a pilin-like protein in competent Bacillus subtilis.
J Biol Chem: 2006, 281(31);21720-21727
[PubMed:16751195] [WorldCat.org] [DOI] (P p)

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

R Provvedi, D Dubnau
ComEA is a DNA receptor for transformation of competent Bacillus subtilis.
Mol Microbiol: 1999, 31(1);271-80
[PubMed:9987128] [WorldCat.org] [DOI] (P p)

G S Inamine, D Dubnau
ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport.
J Bacteriol: 1995, 177(11);3045-51
[PubMed:7768800] [WorldCat.org] [DOI] (P p)

J Hahn, G Inamine, Y Kozlov, D Dubnau
Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA.
Mol Microbiol: 1993, 10(1);99-111
[PubMed:7968523] [WorldCat.org] [DOI] (P p)