Difference between revisions of "ComC"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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** [[ComC]]-[[ComGG]] {{PubMed|9723928}}
 
** [[ComC]]-[[ComGG]] {{PubMed|9723928}}
  
* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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* '''[[Localization]]:'''  
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** integral membrane protein {{PubMed|24164455}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 12:02, 16 March 2014

Gene name comC
Synonyms
Essential no
Product processing protease
Function genetic competence
Gene expression levels in SubtiExpress: comC
Interactions involving this protein in SubtInteract: ComC
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 26 kDa, 9.843
Gene length, protein length 744 bp, 248 aa
Immediate neighbours spoIIB, folC
Sequences Protein DNA DNA_with_flanks
Genetic context
ComC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ComC expression.png















Categories containing this gene/protein

genetic competence, protein secretion, membrane proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU28070

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • processing and translocation of ComGC, ComGD, ComGE and ComGG PubMed
    • Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine (according to Swiss-Prot)
  • Protein family: peptidase A24 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)

Original publications

Inês Chen, Roberta Provvedi, David Dubnau
A macromolecular complex formed by a pilin-like protein in competent Bacillus subtilis.
J Biol Chem: 2006, 281(31);21720-21727
[PubMed:16751195] [WorldCat.org] [DOI] (P p)

Y S Chung, F Breidt, D Dubnau
Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of Bacillus subtilis.
Mol Microbiol: 1998, 29(3);905-13
[PubMed:9723928] [WorldCat.org] [DOI] (P p)

Y S Chung, D Dubnau
ComC is required for the processing and translocation of comGC, a pilin-like competence protein of Bacillus subtilis.
Mol Microbiol: 1995, 15(3);543-51
[PubMed:7783624] [WorldCat.org] [DOI] (P p)

D van Sinderen, A ten Berge, B J Hayema, L Hamoen, G Venema
Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis.
Mol Microbiol: 1994, 11(4);695-703
[PubMed:8196543] [WorldCat.org] [DOI] (P p)

S Mohan, D Dubnau
Transcriptional regulation of comC: evidence for a competence-specific transcription factor in Bacillus subtilis.
J Bacteriol: 1990, 172(7);4064-71
[PubMed:1694528] [WorldCat.org] [DOI] (P p)

S Mohan, J Aghion, N Guillen, D Dubnau
Molecular cloning and characterization of comC, a late competence gene of Bacillus subtilis.
J Bacteriol: 1989, 171(11);6043-51
[PubMed:2553669] [WorldCat.org] [DOI] (P p)