Difference between revisions of "ComA"

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(Extended information on the protein)
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* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
* '''Effectors of protein activity:''' phosphorylation activates DNA binding, DNA-binding activity of ComA is inhibited by [[RapC]] {{PubMed|12950917}} and [[RapD]] {{PubMed|17227471}}
+
* '''Effectors of protein activity:''' phosphorylation activates DNA binding, DNA-binding activity of ComA is inhibited by [[RapC]] {{PubMed|12950917}}, [[RapD]] {{PubMed|17227471}} and [[RapF]] {{PubMed|15968044}}
  
* '''Interactions:''' [[RapC]]-[[ComA]] {{PubMed|12950917}}, [[ComP]]-[[ComA]]
+
* '''Interactions:''' [[RapC]]-[[ComA]] {{PubMed|12950917}}, [[RapF]]-[[ComA]] {{PubMed|15968044}}, [[ComP]]-[[ComA]]
  
 
* '''Localization:''' cytoplasm (according to Swiss-Prot)
 
* '''Localization:''' cytoplasm (according to Swiss-Prot)
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=References=
 
=References=
  
<pubmed>17227471 11133937,2116363,12067344,8387999,19202088, 10094672,8643670,11267663, 16816200, 10447896,12950917, 12270822 </pubmed>
+
<pubmed>17227471 11133937,2116363,12067344,8387999,19202088, 10094672,8643670,11267663, 15968044 16816200, 10447896,12950917, 12270822 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:12, 29 November 2009

  • Description: two-component system response regulator, controls gene expression in response to cell density (concentration of ComX)

Gene name comA
Synonyms srfB, comAA
Essential no
Product two-component system response regulator
Function regulation of genetic competence and quorum sensing
MW, pI 23 kDa, 4.759
Gene length, protein length 642 bp, 214 aa
Immediate neighbours yuxO, comP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ComA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU31680

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: ComA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated by ComP on an Asp residue
  • Cofactor(s):
  • Effectors of protein activity: phosphorylation activates DNA binding, DNA-binding activity of ComA is inhibited by RapC PubMed, RapD PubMed and RapF PubMed
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kassem Hamze, Daria Julkowska, Sabine Autret, Krzysztof Hinc, Krzysztofa Nagorska, Agnieszka Sekowska, I Barry Holland, Simone J Séror
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
Microbiology (Reading): 2009, 155(Pt 2);398-412
[PubMed:19202088] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Yasutaro Fujita
Bacillus subtilis rapD, a direct target of transcription repression by RghR, negatively regulates srfA expression.
FEMS Microbiol Lett: 2007, 268(1);73-80
[PubMed:17227471] [WorldCat.org] [DOI] (P p)

Jennifer M Auchtung, Catherine A Lee, Alan D Grossman
Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.
J Bacteriol: 2006, 188(14);5273-85
[PubMed:16816200] [WorldCat.org] [DOI] (P p)

Cristina Bongiorni, Shu Ishikawa, Sophie Stephenson, Naotake Ogasawara, Marta Perego
Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.
J Bacteriol: 2005, 187(13);4353-61
[PubMed:15968044] [WorldCat.org] [DOI] (P p)

Leighton Core, Marta Perego
TPR-mediated interaction of RapC with ComA inhibits response regulator-DNA binding for competence development in Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1509-22
[PubMed:12950917] [WorldCat.org] [DOI] (P p)

Huang-Mo Sung, Ronald E Yasbin
Adaptive, or stationary-phase, mutagenesis, a component of bacterial differentiation in Bacillus subtilis.
J Bacteriol: 2002, 184(20);5641-53
[PubMed:12270822] [WorldCat.org] [DOI] (P p)

Mireille Ansaldi, Darja Marolt, Tina Stebe, Ines Mandic-Mulec, David Dubnau
Specific activation of the Bacillus quorum-sensing systems by isoprenylated pheromone variants.
Mol Microbiol: 2002, 44(6);1561-73
[PubMed:12067344] [WorldCat.org] [DOI] (P p)

A Yazgan, G Ozcengiz, M A Marahiel
Tn10 insertional mutations of Bacillus subtilis that block the biosynthesis of bacilysin.
Biochim Biophys Acta: 2001, 1518(1-2);87-94
[PubMed:11267663] [WorldCat.org] [DOI] (P p)

P Tortosa, L Logsdon, B Kraigher, Y Itoh, I Mandic-Mulec, D Dubnau
Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system.
J Bacteriol: 2001, 183(2);451-60
[PubMed:11133937] [WorldCat.org] [DOI] (P p)

M Persuh, K Turgay, I Mandic-Mulec, D Dubnau
The N- and C-terminal domains of MecA recognize different partners in the competence molecular switch.
Mol Microbiol: 1999, 33(4);886-94
[PubMed:10447896] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)

M Perego, J A Hoch
Cell-cell communication regulates the effects of protein aspartate phosphatases on the phosphorelay controlling development in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(4);1549-53
[PubMed:8643670] [WorldCat.org] [DOI] (P p)

M Roggiani, D Dubnau
ComA, a phosphorylated response regulator protein of Bacillus subtilis, binds to the promoter region of srfA.
J Bacteriol: 1993, 175(10);3182-7
[PubMed:8387999] [WorldCat.org] [DOI] (P p)

Y Weinrauch, R Penchev, E Dubnau, I Smith, D Dubnau
A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems.
Genes Dev: 1990, 4(5);860-72
[PubMed:2116363] [WorldCat.org] [DOI] (P p)