CitT

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Gene name citT
Synonyms yflQ
Essential no
Product two-component response regulator
Function regulation of citrate uptake
Gene expression levels in SubtiExpress: citT
Interactions involving this protein in SubtInteract: CitT
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 25 kDa, 6.362
Gene length, protein length 678 bp, 226 aa
Immediate neighbours citS, yflP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CitT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CitT expression.png




























Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

The CitT regulon:

The gene

Basic information

  • Locus tag: BSU07590

Phenotypes of a mutant

no growth with citrate as sole carbon source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binding to the promoter of the citM-yflN operon and activation of transcription in the presence of citrate PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated by CitS on an Asp residue
  • Cofactor(s):
  • Effectors of protein activity: phosphorylation likely affects DNA-binding activity

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Guillermo D Repizo, Víctor S Blancato, Pablo D Sender, Juke Lolkema, Christian Magni
Catabolite repression of the citST two-component system in Bacillus subtilis.
FEMS Microbiol Lett: 2006, 260(2);224-31
[PubMed:16842348] [WorldCat.org] [DOI] (P p)

H Yamamoto, M Murata, J Sekiguchi
The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
Mol Microbiol: 2000, 37(4);898-912
[PubMed:10972810] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)