Difference between revisions of "CitT"

From SubtiWiki
Jump to: navigation, search
Line 17: Line 17:
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CitT CitT]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CitT CitT]
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=citT citT]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 6.362   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 6.362   

Revision as of 10:20, 7 January 2014

  • Description: two-component response regulator, regulation of citrate uptake

Gene name citT
Synonyms yflQ
Essential no
Product two-component response regulator
Function regulation of citrate uptake
Gene expression levels in SubtiExpress: citT
Interactions involving this protein in SubtInteract: CitT
Metabolic function and regulation of this protein in SubtiPathways:
citT
MW, pI 25 kDa, 6.362
Gene length, protein length 678 bp, 226 aa
Immediate neighbours citS, yflP
Sequences Protein DNA DNA_with_flanks
Genetic context
CitT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CitT expression.png




























Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

The CitT regulon:

The gene

Basic information

  • Locus tag: BSU07590

Phenotypes of a mutant

no growth with citrate as sole carbon source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binding to the promoter of the citM-yflN operon and activation of transcription in the presence of citrate PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated by CitS on an Asp residue
  • Cofactor(s):
  • Effectors of protein activity: phosphorylation likely affects DNA-binding activity

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Guillermo D Repizo, Víctor S Blancato, Pablo D Sender, Juke Lolkema, Christian Magni
Catabolite repression of the citST two-component system in Bacillus subtilis.
FEMS Microbiol Lett: 2006, 260(2);224-31
[PubMed:16842348] [WorldCat.org] [DOI] (P p)

H Yamamoto, M Murata, J Sekiguchi
The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
Mol Microbiol: 2000, 37(4);898-912
[PubMed:10972810] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)