citS
168
two-component sensor kinase, regulation of citrate uptake
locus
BSU_07580
Molecular weight
59.72 kDa
pI
6.48
function
regulation of citrate uptake
product
two-component sensor kinase
essential
no
synonyms
citS, yflR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3290 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
830,945 832,573
Phenotypes of a mutant
no growth with citrate as sole carbon source [Pubmed|10972810]
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|citT]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
two transmembrane segments
[wiki|PAS domain]
[wiki|Histidine kinase domain] (aa 336-528) (according to UniProt)
Structure
[AF|O34427]
Modification
autophosphorylation on a His residue
Effectors of protein activity
activity may be controlled by interaction with [protein|E4F1B49C26BCB4E3E596406A83CCDCC20AD4A3DB|yflP] [Pubmed|14499931]
[wiki|Localization]
cell membrane (Heterogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227|citS]-[gene|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|citT]-[gene|E4F1B49C26BCB4E3E596406A83CCDCC20AD4A3DB|yflP]
description
[Pubmed|10972810]
Biological materials
Mutant
MGNA-C333 (citS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2331 NBRP B. subtilis, Japan]
BKE07580 ([gene|7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227|citS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGCACCAGCCGTCCTTT, downstream forward: _UP4_AAGGAGAAACAAAGGGGGAA
BKK07580 ([gene|7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227|citS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGCACCAGCCGTCCTTT, downstream forward: _UP4_AAGGAGAAACAAAGGGGGAA
References
Reviews
Original Publications
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Time of last update: 2024-04-26 11:03:48
Author of last update: Melvin.boenninger