Difference between revisions of "CitS"

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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of citrate uptake
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of citrate uptake
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU07580 citS]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07580 citS]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CitS CitS]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CitS CitS]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflS]]'', ''[[citT]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflS]]'', ''[[citT]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12587&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU07580 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU07580 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU07580 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:citS_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:citS_context.gif]]

Revision as of 12:21, 13 May 2013

  • Description: two-component sensor kinase, regulation of citrate uptake

Gene name citS
Synonyms yflR
Essential no
Product two-component sensor kinase
Function regulation of citrate uptake
Gene expression levels in SubtiExpress: citS
Interactions involving this protein in SubtInteract: CitS
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 59 kDa, 6.481
Gene length, protein length 1626 bp, 542 aa
Immediate neighbours yflS, citT
Sequences Protein DNA Advanced_DNA
Genetic context
CitS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CitS expression.png




























Categories containing this gene/protein

utilization of specific carbon sources, protein modification, transcription factors and their control, membrane proteins, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU07580

Phenotypes of a mutant

no growth with citrate as sole carbon source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of CitT
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain
  • Modification: autophosphorylation on a His residue
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Guillermo D Repizo, Víctor S Blancato, Pablo D Sender, Juke Lolkema, Christian Magni
Catabolite repression of the citST two-component system in Bacillus subtilis.
FEMS Microbiol Lett: 2006, 260(2);224-31
[PubMed:16842348] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

H Yamamoto, M Murata, J Sekiguchi
The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
Mol Microbiol: 2000, 37(4);898-912
[PubMed:10972810] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)