citM

168

Mg²⁺-citrate transporter

locus: BSU_07610

Molecular weight: 45.67 kDa

pI: 6.23

Protein length: 433 aa

Gene length: 1302 bp

function: uptake of citrate and magnesium

product: Mg²⁺-citrate transporter

essential: no

synonyms: citM, yflO

List of homologs in different organisms, belongs to COG2851 (Galperin et al., 2021)

Gene

Coordinates: 834,383 → 835,684

Phenotypes of a mutant

• no growth with citrate as sole carbon source [Pubmed|10972810]

Advertisement

The protein

Protein family

• [wiki|CitM transporter family (TC 2.A.11)] (according to UniProt)

Structure

• [AF|P55069]

Paralogous protein(s)

• [protein|71A73C825981E74842FC28D1B05CF85E9F3F5A56|citH]

[wiki|Localization]

• cell membrane (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-C252 (citM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2250 NBRP B. subtilis, Japan]
• GP2244 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''ermC''), available in [wiki|Jörg Stülke]'s lab
• GP2760 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''kan''), available in [wiki|Jörg Stülke]'s lab
• GP2761 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''spec''), available in [wiki|Jörg Stülke]'s lab
• BKE07610 (Δ[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACATCCATTCCCCCTTAAA,  downstream forward: _UP4_TGATAATTCAGGCGTAATGA
• BKK07610 (Δ[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACATCCATTCCCCCTTAAA,  downstream forward: _UP4_TGATAATTCAGGCGTAATGA
• GP2763 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''kan'' ''Δ''''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]''::''spec''), available in [wiki|Jörg Stülke]'s lab

FLAG-tag construct

• GP2767 ''citM-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab

lacZ fusion

• pGP2965 (in [wiki|pAC6]), available in [wiki|Jörg Stülke]'s lab

References

• 16842348,11891560,10972810,11053381,3127276,12375105,8892821,12533460,12670692,11029430,12427932,22900538,24415722