Difference between revisions of "CitM"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07610 citM]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07610 citM]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=citM citM]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 6.227   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 6.227   

Revision as of 10:20, 7 January 2014

  • Description: Mg2+-citrate transporter

Gene name citM
Synonyms yflO
Essential no
Product Mg2+-citrate transporter
Function citrate uptake
Gene expression levels in SubtiExpress: citM
Metabolic function and regulation of this protein in SubtiPathways:
citM
MW, pI 45 kDa, 6.227
Gene length, protein length 1299 bp, 433 aa
Immediate neighbours yflP, yflN
Sequences Protein DNA DNA_with_flanks
Genetic context
CitM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CitM expression.png




























Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon, CitT regulon

The gene

Basic information

  • Locus tag: BSU07610

Phenotypes of a mutant

no growth with citrate as sole carbon source PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: View classification (according to Swiss-Prot)
  • Paralogous protein(s): CitH

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Guillermo D Repizo, Víctor S Blancato, Pablo D Sender, Juke Lolkema, Christian Magni
Catabolite repression of the citST two-component system in Bacillus subtilis.
FEMS Microbiol Lett: 2006, 260(2);224-31
[PubMed:16842348] [WorldCat.org] [DOI] (P p)

Jessica B Warner, Juke S Lolkema
A Crh-specific function in carbon catabolite repression in Bacillus subtilis.
FEMS Microbiol Lett: 2003, 220(2);277-80
[PubMed:12670692] [WorldCat.org] [DOI] (P p)

Jessica B Warner, Christian Magni, Juke S Lolkema
CcpA-independent regulation of expression of the Mg2+ -citrate transporter gene citM by arginine metabolism in Bacillus subtilis.
J Bacteriol: 2003, 185(3);854-9
[PubMed:12533460] [WorldCat.org] [DOI] (P p)

Jessica B Warner, Juke S Lolkema
Growth of Bacillus subtilis on citrate and isocitrate is supported by the Mg2+-citrate transporter CitM.
Microbiology (Reading): 2002, 148(Pt 11);3405-3412
[PubMed:12427932] [WorldCat.org] [DOI] (P p)

Bastiaan P Krom, Henry Huttinga, Jessica B Warner, Juke S Lolkema
Impact of the Mg(2+)-citrate transporter CitM on heavy metal toxicity in Bacillus subtilis.
Arch Microbiol: 2002, 178(5);370-5
[PubMed:12375105] [WorldCat.org] [DOI] (P p)

H Li, A M Pajor
Functional characterization of CitM, the Mg2+-citrate transporter.
J Membr Biol: 2002, 185(1);9-16
[PubMed:11891560] [WorldCat.org] [DOI] (P p)

B P Krom, J B Warner, W N Konings, J S Lolkema
Complementary metal ion specificity of the metal-citrate transporters CitM and CitH of Bacillus subtilis.
J Bacteriol: 2000, 182(22);6374-81
[PubMed:11053381] [WorldCat.org] [DOI] (P p)

J B Warner, B P Krom, C Magni, W N Konings, J S Lolkema
Catabolite repression and induction of the Mg(2+)-citrate transporter CitM of Bacillus subtilis.
J Bacteriol: 2000, 182(21);6099-105
[PubMed:11029430] [WorldCat.org] [DOI] (P p)

H Yamamoto, M Murata, J Sekiguchi
The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
Mol Microbiol: 2000, 37(4);898-912
[PubMed:10972810] [WorldCat.org] [DOI] (P p)

A Boorsma, M E van der Rest, J S Lolkema, W N Konings
Secondary transporters for citrate and the Mg(2+)-citrate complex in Bacillus subtilis are homologous proteins.
J Bacteriol: 1996, 178(21);6216-22
[PubMed:8892821] [WorldCat.org] [DOI] (P p)

P Carlsson, L Hederstedt
Bacillus subtilis citM, the structural gene for dihydrolipoamide transsuccinylase: cloning and expression in Escherichia coli.
Gene: 1987, 61(2);217-24
[PubMed:3127276] [WorldCat.org] [DOI] (P p)