Difference between revisions of "CitG"

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(Biological materials)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
** pGP1122 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]])  
+
** pGP1122 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab)
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 11:47, 9 June 2009

  • Description: fumarase

Gene name citG
Synonyms
Essential no
Product fumarate hydratase
Function TCA cycle
MW, pI 50 kDa, 5.475
Gene length, protein length 1386 bp, 462 aa
Immediate neighbours gerAA, yuxN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CitG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU33040

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate = fumarate + H2O (according to Swiss-Prot)
  • Protein family: Fumarase subfamily (according to Swiss-Prot)
  • Paralogous protein(s): AnsB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1YFE (E.coli)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: constitutive
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP718 (spec), available in Stülke lab
  • Expression vector:
    • pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed