Difference between revisions of "CitA"

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(Biological materials)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
       
+
** pGP2516 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab       
 +
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  

Revision as of 14:46, 8 December 2014

  • Description: minor citrate synthase

Gene name citA
Synonyms citA1
Essential no
Product minor citrate synthase
Function unknown
Gene expression levels in SubtiExpress: citA
Metabolic function and regulation of this protein in SubtiPathways:
citA
MW, pI 40 kDa, 5.778
Gene length, protein length 1098 bp, 366 aa
Immediate neighbours citR, yhdF
Sequences Protein DNA DNA_with_flanks
Genetic context
CitA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CitA expression.png















Categories containing this gene/protein

poorly characterized/ putative enzymes, sporulation proteins

This gene is a member of the following regulons

CitR regulon

The gene

Basic information

  • Locus tag: BSU09440

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + H2O + oxaloacetate = citrate + CoA (according to Swiss-Prot)
  • Protein family: citrate synthase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: CitR: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
    • GP676 (erm), available in the Stülke lab
    • GP1282 (citA::cat), available in Jörg Stülke's lab
    • GP1753 (citR citA::aphA3), the kanamycin resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis, available in Jörg Stülke's lab
  • Expression vector:
    • pGP2516 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References