Difference between revisions of "CitA"
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** GP676 (erm), available in the [[Stülke]] lab | ** GP676 (erm), available in the [[Stülke]] lab | ||
** GP1282 (''[[citA]]''::''cat''), available in [[Jörg Stülke]]'s lab | ** GP1282 (''[[citA]]''::''cat''), available in [[Jörg Stülke]]'s lab | ||
| − | ** GP1753 (''[[citR]] [[citA]]''::''aphA3''), available in [[Jörg Stülke]]'s lab | + | ** GP1753 (''[[citR]] [[citA]]''::''aphA3''), the kanamycin resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
Revision as of 14:08, 8 December 2014
- Description: minor citrate synthase
| Gene name | citA |
| Synonyms | citA1 |
| Essential | no |
| Product | minor citrate synthase |
| Function | unknown |
| Gene expression levels in SubtiExpress: citA | |
| Metabolic function and regulation of this protein in SubtiPathways: citA | |
| MW, pI | 40 kDa, 5.778 |
| Gene length, protein length | 1098 bp, 366 aa |
| Immediate neighbours | citR, yhdF |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
poorly characterized/ putative enzymes, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU09440
Phenotypes of a mutant
Database entries
- BsubCyc: BSU09440
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Acetyl-CoA + H2O + oxaloacetate = citrate + CoA (according to Swiss-Prot)
- Protein family: citrate synthase family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU09440
- Structure: 2C6X
- UniProt: P39119
- KEGG entry: [3]
- E.C. number: 2.3.3.1
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- GP676 (erm), available in the Stülke lab
- GP1282 (citA::cat), available in Jörg Stülke's lab
- GP1753 (citR citA::aphA3), the kanamycin resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis, available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- FLAG-tag construct:
- GP1287 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
