cheD

168

chemoreceptor deaminase, required for methylation of methyl-accepting chemotaxis proteins by [protein|1217807AE3AE3A654BA0DD4AF5ADF1CEE62632B6|cheR], enhances phosphatase activity of [protein|DC9B5446484E42F46EB2B0541192D7D8B4B3AC8F|cheC], required for full [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC] activity

locus: BSU_16460

Molecular weight: 17.86 kDa

pI: 9.35

Protein length: 166 aa

Gene length: 501 bp

function: [wiki|motility and chemotaxis]

product: protein deaminase, [protein|DC9B5446484E42F46EB2B0541192D7D8B4B3AC8F|cheC] activity modulator

essential: no

ec: 3.5.1.44

synonyms: cheD, ylxK

List of homologs in different organisms, belongs to COG1871 (Galperin et al., 2021)

Gene

Coordinates: 1,715,970 → 1,716,470

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

Advertisement

The protein

Catalyzed reaction/ biological activity

• binds chemoreceptors and increases their ability to activate the kinase activity of [protein|6B3B222E56BF0C95A2371CA5208B5522B44D4689|cheA] [Pubmed|23226535]
• H₂O + L-glutaminyl-[protein] --> L-glutamyl-[protein] + NH₄⁺ (according to UniProt)
• deaminates Gln-586, Gln-593, and Gln594 in [protein|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA] [Pubmed|22931217]
• deaminates Gln-609 in [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC] [Pubmed|22931217]

Protein family

• CheD family (single member, according to UniProt)

Structure

• [PDB|2F9Z] (from Thermotoga maritima, 41% identity) [pubmed|16469702]
• [AF|P40404]

Biological materials

Mutant

• 1A861 ( ''cheD''::''cat''), [Pubmed|7893679], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A861&Search=1A861 BGSC]
• 1A863 ( ''cheD''::''cat''), [Pubmed|7893679], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A863&Search=1A863 BGSC]
• DS6868 (marker-less in NCIB3610) [Pubmed|25313396]
• BKE16460 (Δ[gene|BE9373BEB682E5F8B0938AAA4ED9B723B357ABAE|cheD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATAACAACAGCCTCAGTTG,  downstream forward: _UP4_TAATTAAGGTATTAGGGGGA
• BKK16460 (Δ[gene|BE9373BEB682E5F8B0938AAA4ED9B723B357ABAE|cheD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATAACAACAGCCTCAGTTG,  downstream forward: _UP4_TAATTAAGGTATTAGGGGGA

References

Reviews

• 18774298,18990184

Original Publications

• 25313396,26122431,17850253,21515776,22931217,23226535,7893679,15317802,17908686,12011078,8866475,11722727,9194713,14651647,9657996,8157612,15175317,16469702,24386445,25039821,16469702,35638827