Difference between revisions of "CheC"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * not essential for pellicle biofilm formation and mutant has similar fitness to the wild-type strain when competed during pellicle formation {{PubMed|26122431}} | ||
=== Database entries === | === Database entries === | ||
| Line 134: | Line 135: | ||
* '''Mutant:''' | * '''Mutant:''' | ||
** 1A862 (no resistance), {{PubMed|7893679}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A862&Search=1A862 BGSC] | ** 1A862 (no resistance), {{PubMed|7893679}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A862&Search=1A862 BGSC] | ||
| + | ** DS6867 (marker-less in NCIB3610) {{PubMed|25313396}} | ||
| + | ** TB189 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}} | ||
| + | ** TB205 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}} | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| Line 150: | Line 154: | ||
=References= | =References= | ||
| − | <pubmed>7893679,14993307,18774298,8866475,18990184,17908686,14749334,11722727,9194713, 14651647, 9657996,8157612, 15175317,17609139, 16469702, 20080618,17675386,20133180 17850253 21515776 23226535 24386445</pubmed> | + | <pubmed> 25313396, 26122431, 7893679,14993307,18774298,8866475,18990184,17908686,14749334,11722727,9194713, 14651647, 9657996,8157612, 15175317,17609139, 16469702, 20080618,17675386,20133180 17850253 21515776 23226535 24386445</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:53, 2 July 2015
- Description: control of chemotaxis by interacting with CheD, CheY-P phosphatase, inhibition of CheR-mediated methylation of MCPs
| Gene name | cheC |
| Synonyms | ylxJ |
| Essential | no |
| Product | CheY-P phosphatase |
| Function | motility and chemotaxis |
| Gene expression levels in SubtiExpress: cheC | |
| Interactions involving this protein in SubtInteract: CheC | |
| MW, pI | 22 kDa, 4.035 |
| Gene length, protein length | 627 bp, 209 aa |
| Immediate neighbours | cheW, cheD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
protein modification, motility and chemotaxis
This gene is a member of the following regulons
CodY regulon, DegU regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16450
Phenotypes of a mutant
- not essential for pellicle biofilm formation and mutant has similar fitness to the wild-type strain when competed during pellicle formation PubMed
Database entries
- BsubCyc: BSU16450
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cheC family (according to Swiss-Prot)
- Paralogous protein(s):FliY
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: interaction with CheD enhances phosphatase activity towards CheY-P PubMed
Database entries
- BsubCyc: BSU16450
- Structure:
- UniProt: P40403
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: part of the fla-che operon
- Regulation: see fla-che operon
- Regulatory mechanism: see fla-che operon
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
