cheC

168

control of chemotaxis by interacting with [protein|BE9373BEB682E5F8B0938AAA4ED9B723B357ABAE|cheD], [protein|2F0495C7EAB81BDB1F3181677C555537BD2A972F|cheY]-P phosphatase, inhibition of [protein|1217807AE3AE3A654BA0DD4AF5ADF1CEE62632B6|cheR]-mediated methylation of MCPs

locus: BSU_16450

Molecular weight: 22.90 kDa

pI: 4.03

Protein length: 209 aa

Gene length: 630 bp

function: motility and chemotaxis

product: [protein|2F0495C7EAB81BDB1F3181677C555537BD2A972F|cheY]-P phosphatase

essential: no

synonyms: cheC, ylxJ

List of homologs in different organisms, belongs to COG1776 (Galperin et al., 2021)

Gene

Coordinates: 1,715,344 → 1,715,973

Phenotypes of a mutant

• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

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The protein

Catalyzed reaction/ biological activity

• [protein|2F0495C7EAB81BDB1F3181677C555537BD2A972F|cheY]-P phosphatase
• controls binding of [protein|BE9373BEB682E5F8B0938AAA4ED9B723B357ABAE|cheD] to chemoreceptors in response to the levels of [protein|2F0495C7EAB81BDB1F3181677C555537BD2A972F|cheY]-P [Pubmed|23226535]

Protein family

• CheC family (single member, according to UniProt)

Structure

• [PDB|2F9Z] (from Thermotoga maritima, 31% identity) [pubmed|16469702]
• [AF|P40403]

Effectors of protein activity

• interaction with [protein|BE9373BEB682E5F8B0938AAA4ED9B723B357ABAE|cheD] enhances phosphatase activity towards [protein|2F0495C7EAB81BDB1F3181677C555537BD2A972F|cheY]-P [Pubmed|17908686]

Paralogous protein(s)

• [protein|2E73F8DD89F7CFCDE7FAFC58220D8171D0913BAC|fliY]

Biological materials

Mutant

• 1A862 (no resistance), [Pubmed|7893679], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A862&Search=1A862 BGSC]
• DS6867 (marker-less in NCIB3610) [Pubmed|25313396]
• BKE16450 (Δ[gene|DC9B5446484E42F46EB2B0541192D7D8B4B3AC8F|cheC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACTCATGTCATAACCCCTTT,  downstream forward: _UP4_TTTGTCGCCTTAGGTGCATC
• BKK16450 (Δ[gene|DC9B5446484E42F46EB2B0541192D7D8B4B3AC8F|cheC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACTCATGTCATAACCCCTTT,  downstream forward: _UP4_TTTGTCGCCTTAGGTGCATC

References

Reviews

• 30718302

Original Publications

• 25313396,7893679,14993307,18774298,8866475,18990184,17908686,14749334,11722727,9194713,14651647,9657996,8157612,15175317,17609139,16469702,20080618,17675386,20133180,17850253,21515776,23226535,24386445,16469702,35638827