CheA

• Description: two-component sensor kinase, chemotactic signal modulator
  
  

Gene name	cheA
Synonyms	cheN
Essential	no
Product	two-component sensor kinase
Function	chemotactic signal modulator
Gene expression levels in SubtiExpress: cheA
Interactions involving this protein in SubtInteract: CheA
MW, pI	74 kDa, 4.452
Gene length, protein length	2013 bp, 671 aa
Immediate neighbours	cheB, cheW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, motility and chemotaxis, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of CheY and CheB PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:
  • phosphorylation of CheA is stimulated by interaction with the complex of deaminated McpC-CheD PubMed
  • the kinase activity of CheA is activated by chemoreceptors bound to CheD PubMed

• Interactions:
  • CheA-McpB PubMed
  • CheA-CheC PubMed
  • CheA-CheY PubMed

• Localization:
  • membrane (according to Swiss-Prot)
  • localizes to the cell poles PubMed

Database entries

• Structure:

• UniProt: P29072

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: cheA PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:
  • in minimal medium, CheA is present with 2,600 +/- 560 molecules per cell PubMed

Biological materials

• Mutant:
  • 1A797 ( cheA::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Gerald L Hazelbauer, Wing-Cheung Lai
Bacterial chemoreceptors: providing enhanced features to two-component signaling.
Curr Opin Microbiol: 2010, 13(2);124-32
[PubMed:20122866] [WorldCat.org] [DOI] (I p)