Difference between revisions of "CheA"

From SubtiWiki
Jump to: navigation, search
(Extended information on the protein)
Line 61: Line 61:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
  
 
=== Database entries ===
 
=== Database entries ===
Line 145: Line 146:
 
* '''Mutant:'''
 
* '''Mutant:'''
 
** 1A797 ( ''cheA''::''cat''), {{PubMed|1938941}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A797&Search=1A797 BGSC]
 
** 1A797 ( ''cheA''::''cat''), {{PubMed|1938941}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A797&Search=1A797 BGSC]
 +
** DS6887 (marker-less in NCIB3610) {{PubMed|25313396}}
 +
** TB19 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
 +
** TB213 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
Line 157: Line 161:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
* [[Daniel Kearns]]
  
 
=Your additional remarks=
 
=Your additional remarks=
Line 164: Line 169:
 
<pubmed> 20122866  </pubmed>
 
<pubmed> 20122866  </pubmed>
 
==Original publications==
 
==Original publications==
<pubmed>1938941,12123464,17908686,8866475,12094812,10094672,11722727,14651647, 9657996,8157612,15175317 9721285 17850253 11553614 21098025 22931217 21515776 23226535 24386445 25039821</pubmed>
+
<pubmed> 26122431, 25313396, 1938941,12123464,17908686,8866475,12094812,10094672,11722727,14651647, 9657996,8157612,15175317 9721285 17850253 11553614 21098025 22931217 21515776 23226535 24386445 25039821</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:44, 2 July 2015

  • Description: two-component sensor kinase, chemotactic signal modulator

Gene name cheA
Synonyms cheN
Essential no
Product two-component sensor kinase
Function chemotactic signal modulator
Gene expression levels in SubtiExpress: cheA
Interactions involving this protein in SubtInteract: CheA
MW, pI 74 kDa, 4.452
Gene length, protein length 2013 bp, 671 aa
Immediate neighbours cheB, cheW
Sequences Protein DNA DNA_with_flanks
Genetic context
CheA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CheA expression.png















Categories containing this gene/protein

protein modification, transcription factors and their control, motility and chemotaxis, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16430

Phenotypes of a mutant

  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of CheY and CheB PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • autophosphorylation on a His residue
  • Cofactor(s):
  • Effectors of protein activity:
    • phosphorylation of CheA is stimulated by interaction with the complex of deaminated McpC-CheD PubMed
    • the kinase activity of CheA is activated by chemoreceptors bound to CheD PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • in minimal medium, CheA is present with 2,600 +/- 560 molecules per cell PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 705 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1580 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 483 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 188 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 62 PubMed

Biological materials

  • Mutant:
    • 1A797 ( cheA::cat), PubMed, available at BGSC
    • DS6887 (marker-less in NCIB3610) PubMed
    • TB19 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
    • TB213 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Gerald L Hazelbauer, Wing-Cheung Lai
Bacterial chemoreceptors: providing enhanced features to two-component signaling.
Curr Opin Microbiol: 2010, 13(2);124-32
[PubMed:20122866] [WorldCat.org] [DOI] (I p)

Original publications