cdd

168

cytidine deaminase

locus: BSU_25300

Molecular weight: 14.71 kDa

pI: 4.7

Protein length: 136 aa

Gene length: 411 bp

function: pyrimidine interconversion

product: cytidine deaminase

essential: no

ec: 3.5.4.5

synonyms: cdd

List of homologs in different organisms, belongs to COG0295 (Galperin et al., 2021)

Gene

Coordinates: 2,610,927  2,611,337

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The protein

Catalyzed reaction/ biological activity

• cytidine + H⁺ + H₂O --> NH₄⁺ + uridine (according to UniProt)
• 2'-deoxycytidine + H⁺ + H₂O --> 2'-deoxyuridine + NH₄⁺ (according to UniProt)

Protein family

• [wiki|Cytidine and deoxycytidylate deaminase family] (according to UniProt)

[wiki|Domains]

• [wiki|CMP/dCMP-type deaminase domain] (aa 1–128) (according to UniProt)

[wiki|Cofactors]

• Zn²⁺ [Pubmed|10891079]

Structure

• [PDB|1JTK] (complex with inhibitor) [Pubmed|11851403]
• [PDB|1UWZ] (with Arg56Ala substitution) [Pubmed|15147186]
• [PDB|1UX0] (with Arg56Gln substitution) [Pubmed|15147186]
• [PDB|1UX1] (with Cys53His and Arg56Gln substitutions) [Pubmed|15147186]
• [AF|P19079]

Biological materials

Mutant

• BKE25300 ([gene|0E9E9615CE056BEA2B65C494D946EB45F42A3064|cdd]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTACATTCCTCTCTT,  downstream forward: _UP4_GGCGCATTTTCATCGGAGGA
• BKK25300 ([gene|0E9E9615CE056BEA2B65C494D946EB45F42A3064|cdd]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTACATTCCTCTCTT,  downstream forward: _UP4_GGCGCATTTTCATCGGAGGA

References

• 10891079,10493793,11851403,15147186,2526291,10217788,6330116