CdaR

• Description: effector protein controlling CdaA diadenylate cyclase activity
  
  

Gene name	cdaR
Synonyms	ybbR
Essential	no
Product	effector protein controlling CdaA diadenylate cyclase activity
Function	regulation of c-di-AMP synthesis
Gene expression levels in SubtiExpress: cdaR
Interactions involving this protein in SubtInteract: CdaR
Metabolic function and regulation of this protein in SubtiPathways: Murein recycling
MW, pI	52 kDa, 4.719
Gene length, protein length	1449 bp, 483 aa
Immediate neighbours	cdaA, glmM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU01760

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • stimulates CdaA activity upon protein-protein interaction PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CdaA-CdaR, to stimulate CdaA activity PubMed

• Localization:

Database entries

• Structure:

• UniProt: O34659

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: cdaA-cdaR-glmM-glmS PubMed

• Expression browser: cdaR PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: the cdaA-cdaR part of the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:
  • GP999 (cdaR::cat), available in Jörg Stülke's lab
  • GP985 (cdaA-cdaR::cat), available in Jörg Stülke's lab

• Expression vector:
  • IPTG inducible expression of Strep-cdaR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.

• Strep-tag construct:
  • GP1330 (aphA3, based on pGP1460), available in Jörg Stülke's lab
  • GP1380 lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab

• Antibody:

References

Additional publications: PubMed

Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352] [WorldCat.org] [DOI] (I p)

Jun Yin, Paul D Straight, Shaun M McLoughlin, Zhe Zhou, Alison J Lin, David E Golan, Neil L Kelleher, Roberto Kolter, Christopher T Walsh
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase.
Proc Natl Acad Sci U S A: 2005, 102(44);15815-20
[PubMed:16236721] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)