Difference between revisions of "CdaR"

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(Biological materials)
Line 130: Line 130:
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:'''  
+
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab. Respective plasmids: pGP1989 and pGP1992.
  
 
* '''Strep-tag construct:''' GP1330 (aphA3, based on [[pGP1460]]), available in the [[Stülke]] lab
 
* '''Strep-tag construct:''' GP1330 (aphA3, based on [[pGP1460]]), available in the [[Stülke]] lab

Revision as of 16:40, 25 June 2012

  • Description: unknown

Gene name ybbR
Synonyms
Essential no
Product unknown
Function unknown
Metabolic function and regulation of this protein in SubtiPathways:
Murein recycling
MW, pI 52 kDa, 4.719
Gene length, protein length 1449 bp, 483 aa
Immediate neighbours ybbP, glmM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbbR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbbR expression.png
























Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01760

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the ybbP-ybbR part of the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab. Respective plasmids: pGP1989 and pGP1992.
  • Strep-tag construct: GP1330 (aphA3, based on pGP1460), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed